cDNA Clone WNK1

• Plasmid: pcDNA5-FRT/TO-FLAG WNK1 wt
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: FLAG-WNK1 wt
• Genbank: CAC15059.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGTACCATCGATATTGCCACCATGGACTACAAGGACGACGATGACAAGTC
TGGCGGCGCCGCAGAGAAGCAGAGCAGCACTCCCGGTTCCCTGTTCCTCT
CGCCGCCGGCTCCTGCCCCCAAGAATGGCTCCAGCTCCGATTCCTCCGTG
GGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTGACCGGCAGGACCGA
GGAGTACAGGCGCCGCCGCCACACTATGGACAAGGACAGCCGTGGGGCGG
CCGCGACCACTACCACCACTGAGCACCGCTTCTTCCGCCGGAGCGTCATC
TGCGACTCCAATGCCACTGCGCTGGAGCTTCCCGGCCTTCCTCTTTCCCT
GCCCCAGCCCAGCATCCCCGCGGCTGTCCCGCAGAGTGCTCCACCGGAGC
CCCACCGGGAAGAGACCGTGACCGCCACCGCCACTTCCCAGGTAGCCCAG
CAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCCGTCGCGGGCCCTGC
CCCCTCGACTGTCCCCAGCAGTACCAGCAAAGACCGCCCAGTGTCCCAGC
CTAGCCTTGTGGGGAGCAAAGAGGAGCCGCCGCCGGCGAGAAGTGGCAGC
GGCGGCGGCAGCGCCAAGGAGCCACAGGAGGAACGGAGCCAGCAGCAGGA
TGATATCGAAGAGCTGGAGACCAAGGCCGTGGGAATGTCTAACGATGGCC
GCTTTCTCAAGTTTGACATCGAAATCGGCAGAGGCTCCTTTAAGACGGTC
TACAAAGGTCTGGACACTGAAACCACCGTGGAAGTCGCCTGGTGTGAACT
GCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAGATTTAAAGAAGAAG
CTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTGTTAGATTTTATGAT
TCCTGGGAATCCACAGTAAAAGGAAAGAAGTGCATTGTTTTGGTGACTGA
ACTTATGACGTCTGGAACACTTAAAACGTATCTGAAAAGGTTTAAAGTGA
TGAAGATCAAAGTTCTAAGAAGCTGGTGCCGTCAGATCCTTAAAGGTCTT
CAGTTTCTTCATACTCGAACTCCACCTATCATTCACCGCGATCTTAAATG
TGACAACATCTTTATCACCGGCCCTACTGGCTCAGTCAAGATTGGAGACC
TCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGT
ACCCCAGAGTTCATGGCCCCTGAGATGTATGAGGAGAAATATGATGAATC
CGTTGACGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTG
AATATCCTTACTCGGAGTGCCAAAATGCTGCGCAGATCTACCGTCGCGTG
ACCAGTGGGGTGAAGCCAGCCAGTTTTGACAAAGTAGCAATTCCTGAAGT
GAAGGAAATTATTGAAGGATGCATACGACAAAACAAAGATGAAAGATATT
CCATCAAAGACCTTTTGAACCATGCCTTCTTCCAAGAGGAAACAGGAGTA
CGGGTAGAATTAGCAGAAGAAGATGATGGAGAAAAAATAGCCATAAAATT
ATGGCTACGTATTGAAGATATTAAGAAATTAAAGGGAAAATACAAAGATA
ATGAAGCTATTGAGTTTTCTTTTGATTTAGAGAGAGATGTCCCAGAAGAT
GTTGCACAAGAAATGGTAGAGTCTGGGTATGTCTGTGAAGGTGATCACAA
GACCATGGCTAAAGCTATCAAAGACAGAGTATCATTAATTAAGAGGAAAC
GAGAGCAGCGGCAGTTGGTACGGGAGGAGCAAGAAAAAAAAAAGCAGGAA
GAGAGCAGTCTCAAACAGCAGGTAGAACAATCCAGTGCTTCCCAGACAGG
AATCAAGCAGCTCCCTTCTGCTAGCACCGGCATACCTACTGCTTCTACCA
CTTCAGCTTCAGTTTCTACACAAGTAGAACCTGAAGAACCTGAGGCAGAT
CAACATCAGCAACTACAGTACCAGCAACCCAGTATATCTGTGTTATCTGA
TGGGACGGTTGACAGTGGTCAGGGATCCTCTGTCTTCACAGAATCTCGAG
TGAGCAGCCAACAGACAGTTTCATATGGTTCCCAACATGAACAGGCACAT
TCTACAGGCACAGTCCCAGGGCATATACCTTCTACTGTCCAAGCACAGTC
TCAGCCCCATGGGGTATATCCACCCTCAAGTGTGGCACAGGGGCAGAGCC
AGGGTCAGCCATCCTCAAGTAGCTTAACAGGGGTTTCATCTTCCCAACCC
ATACAACATCCTCAGCAGCAGCAGGGAATACAGCAGACAGCCCCTCCTCA
ACAGACAGTGCAGTATTCACTTTCACAGACATCAACCTCCAGTGAGGCCA
CTACTGCACAGCCAGTGAGTCAACCTCAAGCTCCACAAGTCTTGCCTCAA
GTATCAGCTGGAAAACAGCTTCCAGTTTCCCAGCCAGTACCAACTATCCA
AGGCGAACCTCAGATCCCAGTTGCGACACAACCCTCGGTTGTTCCAGTCC
ACTCTGGTGCTCATTTCCTTCCAGTGGGACAGCCGCTCCCTACTCCCTTG
CTCCCTCAGTACCCTGTCTCTCAGATTCCCATATCAACTCCTCATGTGTC
TACGGCTCAGACAGGTTTCTCATCCCTTCCCATCACAATGGCAGCTGGCA
TTACTCAGCCTCTGCTCACGTTGGCTTCATCTGCTACAACAGCTGCGATC
CCGGGGGTATCAACTGTGGTTCCTAGTCAGCTTCCAACCCTTCTGCAGCC
TGTGACTCAGCTGCCAAGTCAGGTTCACCCACAGCTCCTACAACCAGCAG
TTCAGTCCATGGGAATACCAGCTAACCTTGGACAAGCTGCTGAGGTTCCA
CTTTCCTCTGGAGATGTTCTGTACCAGGGCTTCCCACCTCGACTGCCACC
ACAGTACCCAGGAGATTCAAATATTGCTCCCTCTTCCAACGTGGCTTCTG
TTTGCATCCATTCTACAGTCCTATCCCCTCCCATGCCGACAGAAGTACTG
GCTACACCTGGGTACTTTCCCACAGTGGTGCAGCCTTATGTGGAATCAAA
TCTTTTAGTTCCTATGGGTGGTGTAGGAGGACAGGTTCAAGTGTCCCAGC
CAGGAGGGAGTTTAGCACAAGCCCCCACTACATCCTCCCAGCAAGCAGTT
TTGGAGAGTACTCAGGGAGTCTCTCAGGTTGCTCCTGCAGAGCCAGTTGC
AGTAGCACAGCCCCAAGCTACCCAGCCGACCACTTTGGCTTCCTCTGTAG
ACAGTGCACATTCAGATGTTGCTTCAGGTATGAGTGATGGCAATGAGAAC
GTCCCATCTTCCAGTGGAAGGCATGAAGGAAGAACTACAAAACGGCATTA
CCGAAAATCTGTAAGGAGTCGCTCTCGACATGAAAAAACTTCACGCCCAA
AATTAAGAATTTTGAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGT
CAATTAGAGACTCATAATAGGAAAATGGTTACATTCAAATTTGACCTAGA
TGGTGACAACCCCGAGGAGATAGCAACAATTATGGTGAACAATGACTTTA
TTCTAGCAATAGAGAGAGAGTCGTTTGTGGATCAAGTGCGAGAAATTATT
GAAAAAGCTGATGAAATGCTCAGTGAGGATGTCAGTGTGGAACCAGAGGG
TGATCAGGGATTGGAGAGTCTACAAGGAAAGGATGACTATGGCTTTTCAG
GTTCTCAGAAGCTTGAAGGAGAGTTCAAACAACCAATTCCTGCGTCTTCC
ATGCCACAGCAAATAGGCATTCCTACCAGTTCTTTAACTCAAGTTGTTCA
TTCTGCGGGAAGGCGGTTTATAGTGAGTCCTGTGCCAGAAAGCCGATTAC
GAGAATCAAAAGTTTTCCCCAGTGAAATAACAGATACAGTTGCTGCCTCT
ACAGCTCAGAGCCCTGGAATGAACTTGTCTCACTCTGCATCATCCCTTAG
TCTACAACAGGCCTTTTCTGAACTTAGACGTGCCCAAATGACAGAAGGAC
CCAACACAGCACCTCCAAACTTTAGTCATACAGGACCAACATTTCCAGTA
GTACCTCCTTTCTTAAGTAGCATTGCTGGAGTCCCAACCACAGCAGCAGC
CACAGCACCAGTCCCTGCAACAAGCAGCCCTCCTAATGACATTTCCACAT
CAGTAATTCAGTCTGAGGTTACAGTGCCCACTGAAGAGGGGATTGCTGGA
GTTGCCACCAGCACAGGTGTGGTAACTTCGGGTGGTCTCCCCATACCACC
TGTGTCTGAATCACCAGTACTTTCCAGCGTAGTTTCAAGTATCACAATAC
CTGCAGTTGTCTCAATATCTACTACATCCCCGTCACTTCAAGTCCCCACA
TCCACATCTGAGATCGTTGTTTCTAGTACAGCACTGTATCCTTCAGTAAC
AGTTTCAGCAACTTCAGCCTCTGCAGGGGGCAGTACTGCTACCCCAGGTC
CTAAGCCTCCAGCTGTAGTATCTCAGCAGGCAGCAGGCAGCACTACTGTG
GGAGCCACATTAACATCAGTTTCTACCACCACTTCATTCCCAAGCACAGC
TTCACAGCTGTCCATTCAGCTTAGCAGCAGTACTTCTACTCCTACTTTAG
CTGAAACCGTGGTAGTTAGCGCACACTCACTAGATAAGACATCTCATAGC
AGTACAACTGGATTGGCTTTCTCCCTCTCTGCACCATCTTCCTCTTCCTC
TCCTGGAGCAGGAGTGTCTAGTTATATTTCTCAGCCTGGTGGGCTGCATC
CTTTGGTCATTCCATCAGTGATAGCTTCTACTCCTATTCTTCCCCAAGCA
GCAGGACCTACTTCTACACCTTTATTACCCCAAGTACCTAGTATCCCACC
CTTGGTACAGCCTGTTGCCAATGTGCCTGCTGTACAGCAGACACTAATTC
ATAGTCAGCCTCAACCAGCTTTGCTTCCCAACCAGCCCCATACTCATTGT
CCTGAAGTAGATTCTGATACACAACCCAAAGCTCCTGGAATTGATGACAT
AAAGACTCTAGAAGAAAAGCTGCGGTCTCTGTTCAGTGAACACAGCTCAT
CTGGAGCTCAGCATGCCTCTGTCTCACTGGAGACCTCACTAGTCATAGAG
AGCACTGTCACACCAGGCATCCCAACTACTGCTGTTGCACCAAGCAAACT
CCTGACTTCTACCACAAGTACTTGCTTACCACCAACCAATTTACCACTAG
GAACAGTTGCTTTGCCAGTTACACCAGTGGTCACACCTGGGCAAGTTTCT
ACCCCAGTCAGCACTACTACATCAGGAGTGAAACCTGGAACTGCTCCCTC
CAAGCCACCTCTAACTAAGGCTCCGGTGCTGCCAGTGGGTACTGAACTTC
CAGCAGGTACTCTACCCAGCGAGCAGCTGCCACCTTTTCCAGGACCTTCT
CTAACCCAGTCCCAGCAACCTCTAGAGGATCTTGATGCTCAATTGAGAAG
AACACTTAGTCCAGAGATTATCACAGTGACTTCTGCGGTTGGTCCTGTGT
CCATGGCGGCTCCAACAGCAATCACAGAAGCAGGAACACAGCCTCAGAAG
GGTGTTTCTCAAGTCAAAGAAGGCCCTGTCCTAGCGACTAGTTCAGGAGC
TGGTGTTTTTAAGATGGGACGATTTCAGGTTTCTGTTGCAGCAGACGGTG
CCCAGAAAGAGGGTAAAAATAAGTCAGAAGATGCAAAGTCTGTTCATTTT
GAATCCAGCACCTCAGAGTCCTCAGTGCTATCAAGTAGTAGTCCAGAGAG
TACCTTGGTGAAACCAGAGCCGAATGGCATAACCATCCCTGGTATCTCTT
CAGATGTGCCAGAGAGTGCCCACAAAACTACTGCCTCAGAGGCAAAGTCA
GACACTGGGCAGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGC
AAACAAAGTGGGTCGTTTCTCTGTATCAAAAACTGAGGACAAGATCACTG
ACACAAAGAAAGAAGGACCAGTGGCATCTCCTCCTTTTATGGATTTGGAA
CAAGCTGTTCTTCCTGCTGTGATACCAAAGAAAGAGAAGCCTGAACTGTC
AGAGCCTTCACATCTAAATGGGCCGTCTTCTGACCCGGAGGCCGCTTTTT
TAAGTAGGGATGTGGATGATGGTTCCGGTAGTCCACACTCGCCCCATCAG
CTGAGCTCAAAGAGCCTTCCTAGCCAGAATCTAAGTCAAAGCCTTAGTAA
TTCATTTAACTCCTCTTACATGAGTAGCGACAATGAGTCAGATATCGAAG
ATGAAGACTTAAAGTTAGAGCTGCGACGACTACGAGATAAACATCTCAAA
GAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTA
TACCAAACTGGGCAAGGTGCCCCCTGCTGTTATTATTCCCCCAGCTGCTC
CCCTTTCAGGGAGAAGACGACGACCCACTAAAAGCAAAGGCAGCAAATCT
AGTCGAAGCAGTTCCTTGGGGAATAAAAGCCCCCAGCTTTCAGGTAACCT
GTCTGGTCAGAGTGCAGCTTCAGTCTTGCACCCCCAGCAGACCCTCCACC
CTCCTGGCAACATCCCAGAGTCCGGGCAGAATCAGCTGTTACAGCCCCTT
AAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTCACCAGTGATGG
TGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGAACCAGCAGCA
CAAACACTGTTGGGGCAACAGTGAACAGCCAAGCCGCCCAAGCTCAGCCT
CCTGCCATGACGTCCAGCAGGAAGGGCACATTCACAGATGACTTGCACAA
GTTGGTAGACAATTGGGCCCGAGATGCCATGAATCTCTCAGGCAGGAGAG
GAAGCAAAGGGCACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTC
TCTGCACCTGGGCAACTGTGCATCTCCATGACCTCGAACCTGGGTGGCTC
TGCCCCCATCTCTGCAGCATCAGCTACCTCTCTAGGTCACTTCACCAAGT
CTATGTGCCCCCCACAGCAGTATGGCTTTCCAGCTACCCCATTTGGCGCT
CAATGGAGTGGGACGGGTGGCCCAGCACCACAGCCACTTGGCCAGTTCCA
ACCTGTGGGAACTGCCTCCTTGCAGAATTTCAACATCAGCAATTTGCAGA
AATCCATCAGCAACCCCCCAGGCTCCAACCTGCGGACCACTTAGATCGAT
GTCGAC

Amino Acid Sequence

MDYKDDDDKSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAA
AADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATA
LELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAA
PGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKE
PQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTE
TTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVK
GKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRT
PPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAP
EMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPA
SFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEE
DDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVE
SGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQ
VEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQY
QQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPG
HIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQ
QGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQL
PVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVS
QIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVV
PSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVL
YQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFP
TVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGV
SQVAPAEPVAVAQPQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGR
HEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNR
KMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEML
SEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGI
PTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGM
NLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSS
IAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGV
VTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVV
SSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSV
STTTSFPSTASQLSIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAF
SLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTP
LLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDT
QPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGI
PTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTT
SGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQP
LEDLDAQLRRTLSPEIITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKE
GPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSES
SVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTK
VGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAV
IPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLP
SQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQS
RQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLG
NKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSD
NLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSR
KGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLC
ISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGG
PAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU13374
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.