cDNA Clone 14-3-3 beta

• Synonyms: YWHAB
• Plasmid: pET28a 14-3-3 beta
• Parent Plasmid: pET28a original
• Expressed: 6His-14-3-3 beta
• Genbank: NP_647539
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACAATGGATAAAAGTGAGCTGGTACAGAAAGCCAAACTCGC
TGAGCAGGCTGAGCGATATGATGATATGGCTGCAGCCATGAAGGCAGTCA
CAGAACAGGGGCATGAACTCTCCAACGAAGAGAGAAATCTGCTCTCTGTT
GCCTACAAGAATGTGGTAGGCGCCCGCCGCTCTTCCTGGCGTGTCATCTC
CAGCATTGAGCAGAAAACAGAGAGGAATGAGAAGAAGCAGCAGATGGGCA
AAGAGTACCGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGAT
GTTCTGGAGCTGTTGGACAAATATCTTATTCCCAATGCTACACAACCAGA
AAGTAAGGTGTTCTACTTGAAAATGAAAGGAGATTATTTTAGGTATCTTT
CTGAAGTGGCATCTGGAGACAACAAACAAACCACTGTGTCGAACTCCCAG
CAGGCTTACCAGGAAGCATTTGAAATTAGTAAGAAAGAAATGCAGCCTAC
ACACCCAATTCGTCTTGGTCTGGCACTAAATTTCTCAGTCTTTTACTATG
AGATTCTAAACTCTCCTGAAAAGGCCTGTAGCCTGGCAAAAACGGCATTT
GATGAAGCAATTGCTGAATTGGATACGCTGAATGAAGAGTCTTATAAAGA
CAGCACTCTGATCATGCAGTTACTTAGGGACAATCTCACTCTGTGGACAT
CGGAAAACCAGGGAGACGAAGGAGACGCTGGGGAGGGAGAGAACTAAGCG
GCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMTMDKSELVQKAKLAE
QAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISS
IEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPES
KVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTH
PIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDS
TLIMQLLRDNLTLWTSENQGDEGDAGEGEN*AA

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8261
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; ; Estimated MW of expressed protein : 31625 Daltons