cDNA Clone 14-3-3 theta

• Plasmid: pET28a 14-3-3 theta
• Parent Plasmid: pET28a original
• Expressed: 6His-14-3-3 theta
• Genbank: NP_006817.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAGAAGACTGAGCTGATCCAGAAGGCCAAGCTGGCCGAGCA
GGCCGAGCGCTACGACGACATGGCCACCTGCATGAAGGCAGTGACCGAGC
AGGGCGCCGAGCTGTCCAACGAGGAGCGCAACCTGCTCTCCGTGGCCTAC
AAGAACGTGGTCGGGGGCCGCAGGTCCGCCTGGAGGGTCATCTCTAGCAT
CGAGCAGAAGACCGACACCTCCGACAAGAAGTTGCAGCTGATTAAGGACT
ATCGGGAGAAAGTGGAGTCCGAGCTGAGATCCATCTGCACCACGGTGCTG
GAATTGTTGGATAAATATTTAATAGCCAATGCAACTAATCCAGAGAGTAA
GGTCTTCTATCTGAAAATGAAGGGTGATTACTTCCGGTACCTTGCTGAAG
TTGCGTGTGGTGATGATCGAAAACAAACGATAGATAATTCCCAAGGAGCT
TACCAAGAGGCATTTGATATAAGCAAGAAAGAGATGCAACCCACACACCC
AATCCGCCTGGGGCTTGCTCTTAACTTTTCTGTATTTTACTATGAGATTC
TTAATAACCCAGAGCTTGCCTGCACGCTGGCTAAAACGGCTTTTGATGAG
GCCATTGCTGAACTTGATACACTGAATGAAGACTCATACAAAGACAGCAC
CCTCATCATGCAGTTGCTTAGAGACAACCTAACACTTTGGACATCAGACA
GTGCAGGAGAAGAATGTGATGCGGCAGAAGGGGCTGAAAACTAAGCGGCC
GC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMEKTELIQKAKLAEQA
ERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIE
QKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKV
FYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPI
RLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTL
IMQLLRDNLTLWTSDSAGEECDAAEGAEN*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8265
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; Estimated MW of expressed protein : 31306 Daltons