cDNA Clone 14-3-3 theta

• Plasmid: pGEX6P-1-14-3-3 theta
• Parent Plasmid: pGEX6P1
• Expressed: GST-14-3-3 theta
• Genbank: NP_006817.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGAAGACTGAGCTGATCCAGAAGGCCAAGCTGGCCGAGCA
GGCCGAGCGCTACGACGACATGGCCACCTGCATGAAGGCAGTGACCGAGC
AGGGCGCCGAGCTGTCCAACGAGGAGCGCAACCTGCTCTCCGTGGCCTAC
AAGAACGTGGTCGGGGGCCGCAGGTCCGCCTGGAGGGTCATCTCTAGCAT
CGAGCAGAAGACCGACACCTCCGACAAGAAGTTGCAGCTGATTAAGGACT
ATCGGGAGAAAGTGGAGTCCGAGCTGAGATCCATCTGCACCACGGTGCTG
GAATTGTTGGATAAATATTTAATAGCCAATGCAACTAATCCAGAGAGTAA
GGTCTTCTATCTGAAAATGAAGGGTGATTACTTCCGGTACCTTGCTGAAG
TTGCGTGTGGTGATGATCGAAAACAAACGATAGATAATTCCCAAGGAGCT
TACCAAGAGGCATTTGATATAAGCAAGAAAGAGATGCAACCCACACACCC
AATCCGCCTGGGGCTTGCTCTTAACTTTTCTGTATTTTACTATGAGATTC
TTAATAACCCAGAGCTTGCCTGCACGCTGGCTAAAACGGCTTTTGATGAG
GCCATTGCTGAACTTGATACACTGAATGAAGACTCATACAAAGACAGCAC
CCTCATCATGCAGTTGCTTAGAGACAACCTAACACTTTGGACATCAGACA
GTGCAGGAGAAGAATGTGATGCGGCAGAAGGGGCTGAAAACTAAGCGGCC
GC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEKTELIQKAKLAEQAERY
DDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKT
DTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYL
KMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLG
LALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQ
LLRDNLTLWTSDSAGEECDAAEGAEN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8299
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 54586 Daltons