cDNA Clone VPS15

• Plasmid: pGEX6P-2-VPS15 1-680
• Parent Plasmid: pGEX6P2
• Expressed: GST-VPS15 1-680
• Genbank: NP_055417
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCT
TTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATAAAA
GCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGAA
GGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCCTTT
AACCAGCTATAAACAAGAGCTGGAGGAACTGAAAATCAGGCTTAATTCTG
CACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAGCATCTGAGAAA
GCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCAT
CAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCC
AGATCCTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCATGGG
GACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCTTCT
AACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCGG
CAGATTTCAATTATTTCTTTGACACATCACGGAGGAGAACTTGCTATATT
GCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTAGAATA
TATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAA
CAAGAGGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTG
ATAGCTGAGCTTTTTACAGAAGGTGTACCATTATTTGATCTCTCTCAACT
TTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAAA
TTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAG
CCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGTGGCAA
TGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCCAGT
TTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGG
AAGGATTTGGGCAACATTATTCACAATCTCTGTGGACATGATCTGCCAGA
AAAAGCCGAAGGAGAGCCTAAGGAAAATGGGCTGGTTATCTTGGTATCTG
TTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGCT
GCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCT
TTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTGTTC
CTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCTCTC
GTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACATTCT
GCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTAG
CCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTCCTG
GAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATGA
AGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCC
AAGCCTTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGTGAC
CCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGAATAACACGGCT
GTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCACA
TGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTT
TTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCTC
AATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTTG
TCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTG
CTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATTGCCCCCTTCCT
GTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGGATTTATCACAT
AAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMGNQLAGI
APSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQ
DPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRD
NLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTS
WNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMF
ATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPL
FDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYL
KQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHNLC
GHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPR
LSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINI
YPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLNME
NDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLME
NGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYV
GWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEFAS
DIAPFLCHPNLWIRYGAVGFIT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8564
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 153006 Daltons