cDNA Clone Ubiquitin

• Plasmid: pET15b6P Ubiquitin K63wt
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His Ubiquitin K63wt
• Tag: 6His

Sequence of Insert

GGATCTCAAATCTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAGGATCCAAGACA
GGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSQIFVRTLTGRTITLEVEPSDTIENVRA
RIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQKESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8744
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for bacterial expression.