cDNA Clone Ubiquitin

• Plasmid: pGEX6P-1-FLAG Ubiquitin K29wt K33wt
• Parent Plasmid: pGEX6P1
• Expressed: GST-FLAG Ubiquitin K29wt K33wt
• Genbank: P62988
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGGATCTGCCACCATGGACTACAAGGACGACGATGACAAGCAAAT
CTTCGTGAGGACCCTGACTGGTAGGACCATCACTCTCGAAGTGGAGCCGA
GTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACAAGGAAGGCATC
CCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAGCTGGAAGATGG
ACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCTGCACCTGG
TCCTCCGTCTCAGAGGTGGGTGATAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFGSATMDYKDDDDKQIF
VRTLTGRTITLEVEPSDTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGR
TLSDYNIQRESTLHLVLRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8990
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/FLAG for bacterial expression.