cDNA Clone CIKS

• Plasmid: pEBG6P-CIKS
• Parent Plasmid: pEBG6P
• Expressed: GST-CIKS
• Genbank: NP_671733.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCTCCTCAGCTTCAAGAAACTAGAATGAACCGAAGCATTCC
TGTGGAGGTTGATGAATCAGAACCATACCCAAGTCAGTTGCTGAAACCAA
TCCCAGAATATTCCCCGGAAGAGGAATCAGAACCACCTGCTCCAAATATA
AGGAACATGGCACCCAACAGCTTGTCTGCACCCACAATGCTTCACAATTC
CTCCGGAGACTTTTCTCAAGCTCACTCAACCCTGAAACTTGCAAATCACC
AGCGGCCTGTATCCCGGCAGGTCACCTGCCTGCGCACTCAAGTTCTGGAG
GACAGTGAAGACAGTTTCTGCAGGAGACACCCAGGCCTGGGCAAAGCTTT
CCCTTCTGGGTGCTCTGCAGTCAGCGAGCCTGCGTCTGAGTCTGTGGTTG
GAGCCCTCCCTGCAGAGCATCAGTTTTCATTTATGGAAAAACGTAATCAA
TGGCTGGTATCTCAGCTTTCAGCGGCTTCTCCTGACACTGGCCATGACTC
AGACAAATCAGACCAAAGTTTACCTAATGCCTCAGCAGACTCCTTGGGCG
GTAGCCAGGAGATGGTGCAACGGCCCCAGCCTCACAGGAACCGAGCAGGC
CTGGATCTGCCAACCATAGACACGGGATATGATTCCCAGCCCCAGGATGT
CCTGGGCATCAGGCAGCTGGAAAGGCCCCTGCCCCTCACCTCCGTGTGTT
ACCCCCAGGACCTCCCCAGACCTCTCAGGTCCAGGGAGTTCCCTCAGTTT
GAACCTCAGAGGTATCCAGCATGTGCACAGATGCTGCCTCCCAATCTTTC
CCCACATGCTCCATGGAACTATCATTACCATTGTCCTGGAAGTCCCGATC
ACCAGGTGCCATATGGCCATGACTACCCTCGAGCAGCCTACCAGCAAGTG
ATCCAGCCGGCTCTGCCTGGGCAGCCCCTGCCTGGAGCCAGTGTGAGAGG
CCTGCACCCTGTGCAGAAGGTTATCCTGAATTATCCCAGCCCCTGGGACC
AAGAAGAGAGGCCCGCACAGAGAGACTGCTCCTTTCCGGGGCTTCCAAGG
CACCAGGACCAGCCACATCACCAGCCACCTAATAGAGCTGGTGCTCCTGG
GGAGTCCTTGGAGTGCCCTGCAGAGCTGAGACCACAGGTTCCCCAGCCTC
CGTCCCCAGCTGCTGTGCCTAGACCCCCTAGCAACCCTCCAGCCAGAGGA
ACTCTAAAAACAAGCAATTTGCCAGAAGAATTGCGGAAAGTCTTTATCAC
TTATTCGATGGACACAGCTATGGAGGTGGTGAAATTCGTGAACTTTTTGT
TGGTAAATGGCTTCCAAACTGCAATTGACATATTTGAGGATAGAATCCGA
GGCATTGATATCATTAAATGGATGGAGCGCTACCTTAGGGATAAGACCGT
GATGATAATCGTAGCAATCAGCCCCAAATACAAACAGGACGTGGAAGGCG
CTGAGTCGCAGCTGGACGAGGATGAGCATGGCTTACATACTAAGTACATT
CATCGAATGATGCAGATTGAGTTCATAAAACAAGGAAGCATGAATTTCAG
ATTCATCCCTGTGCTCTTCCCAAATGCTAAGAAGGAGCATGTGCCCACCT
GGCTTCAGAACACTCATGTCTACAGCTGGCCCAAGAATAAAAAAAACATC
CTGCTGCGGCTGCTGAGAGAGGAAGAGTATGTGGCTCCTCCACGGGGGCC
TCTGCCCACCCTTCAGGTGGTTCCCTTGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPPQLQETRMNRS
IPVEVDESEPYPSQLLKPIPEYSPEEESEPPAPNIRNMAPNSLSAPTMLH
NSSGDFSQAHSTLKLANHQRPVSRQVTCLRTQVLEDSEDSFCRRHPGLGK
AFPSGCSAVSEPASESVVGALPAEHQFSFMEKRNQWLVSQLSAASPDTGH
DSDKSDQSLPNASADSLGGSQEMVQRPQPHRNRAGLDLPTIDTGYDSQPQ
DVLGIRQLERPLPLTSVCYPQDLPRPLRSREFPQFEPQRYPACAQMLPPN
LSPHAPWNYHYHCPGSPDHQVPYGHDYPRAAYQQVIQPALPGQPLPGASV
RGLHPVQKVILNYPSPWDQEERPAQRDCSFPGLPRHQDQPHHQPPNRAGA
PGESLECPAELRPQVPQPPSPAAVPRPPSNPPARGTLKTSNLPEELRKVF
ITYSMDTAMEVVKFVNFLLVNGFQTAIDIFEDRIRGIDIIKWMERYLRDK
TVMIIVAISPKYKQDVEGAESQLDEDEHGLHTKYIHRMMQIEFIKQGSMN
FRFIPVLFPNAKKEHVPTWLQNTHVYSWPKNKKNILLRLLREEEYVAPPR
GPLPTLQVVPL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12264
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 92072 Daltons