cDNA Clone CERK

• Plasmid: pET28 CERK
• Parent Plasmid: pET28a original
• Expressed: 6His CERK
• Genbank: AAH67255
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTTCAGCGAGGTGCTGCACGGTCTGATTGGGAGGACGCAGAG
GAGCGCCGGGGTCGACCAGAACCACCCCCGGGCTGTGCTGGTCCCCAGTA
GCCTCCGGATTGGAATCATTCCCGCAGGGTCAACGGACTGCGTGTGTTAC
TCCACCGTGGGCACCAGCGACGCAGAAACCTCGGCGCTGCATATCGTTGT
TGGGGACTCGCTGGCCATGGATGTGTCCTCAGTCCACCACAACAGCACAC
TCCTTCGCTACTCCGTGTCCCTGCTGGGCTACGGCTTCTACGGGGACATC
ATCAAGGACAGTGAGAAGAAACGGTGGTTGGGTCTTGCCAGATACGACTT
TTCAGGTTTAAAGACCTTCCTCTCCCACCACTGCTATGAAGGGACAGTGT
CCTTCCTCCCTGCACAACACACGGTGGGATCTCCAAGGGATAGGAAGCCC
TGCCGGGCAGGATGCTTTGTTTGCAGGCAAAGCAAGCAGCAGCTGGAGGA
GGAGCAGAAGAAAGCACTGTATGGTTTGGAAGCTGCGGAGGACGTGGAGG
AGTGGCAAGTCGTCTGTGGGAAGTTTCTGGCCATCAATGCCACAAACATG
TCCTGTGCTTGTCGCCGGAGCCCCAGGGGCCTCTCCCCGGCTGCCCACTT
GGGAGACGGGTCTTCTGACCTCATCCTCATCCGGAAATGCTCCAGGTTCA
ATTTTCTGAGATTTCTCATCAGGCACACCAACCAGCAGGACCAGTTTGAC
TTCACTTTTGTTGAAGTTTATCGCGTCAAGAAATTCCAGTTTACGTCGAA
GCACATGGAGGATGAGGACAGCGACCTCAAGGAGGGGGGGAAGAAGCGCT
TTGGGCACATTTGCAGCAGCCACCCCTCCTGCTGCTGCACCGTCTCCAAC
AGCTCCTGGAACTGCGACGGGGAGGTCCTGCACAGCCCTGCCATCGAGGT
CAGAGTCCACTGCCAGCTGGTTCGACTCTTTGCACGAGGAATTGAAGAGA
ATCCGAAGCCAGACTCACACAGCTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMFSEVLHGLIGRTQRS
AGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVG
DSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFS
GLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAGCFVCRQSKQQLEEE
QKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCACRRSPRGLSPAAHLG
DGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFVEVYRVKKFQFTSKH
MEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHSPAIEVR
VHCQLVRLFARGIEENPKPDSHS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU12307
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 41323 Daltons; Parental 49270