cDNA Clone VPS34

• Plasmid: pGEX6P-2-VPS34 D761A
• Parent Plasmid: pGEX6P2
• Expressed: GST-VPS34 D761A
• Genbank: NP_002638
• Tag: GST

Sequence of Insert

GCGGCCGCGATGGGGGAAGCAGAGAAGTTTCACTACATCTATAGTTGTGA
CCTGGATATCAACGTCCAGCTTAAGATAGGAAGCTTGGGAGGGAAGAGAG
AACAAAAGAGTTATAAAGCTGTCCTGGAAGACCCAATGTTGAAGTTCTCA
GGACTATATCAAGAGACATGCTCTGATCTTTATGTTACTTGTCAAGTTTT
TGCAGAAGGGAAGCCTTTGGCCTTGCCAGTGAGAACATCCTACAAAGCAT
TTAGTACAAGATGGAACTGGAATGAATGGCTGAAACTACCAGTAAAATAC
CCTGACCTGCCCAGGAATGCCCAAGTGGCCCTCACCATATGGGATGTGTA
TGGTCCCGGAAAAGCAGTGCCTGTAGGAGGAACAACGGTTTCGCTCTTTG
GAAAATACGGCATGTTTCGCCAAGGGATGCATGACTTGAAAGTCTGGCCT
AATGTAGAAGCAGATGGATCAGAACCCACAAAAACTCCTGGCAGAACAAG
TAGCACTCTCTCAGAAGATCAGATGAGCCGTCTTGCCAAGCTCACCAAAG
CTCATCGACAAGGACACATGGTGAAAGTAGATTGGCTGGATAGATTGACA
TTTAGAGAAATAGAAATGATAAATGAGAGTGAAAAACGAAGTTCTAATTT
CATGTACCTGATGGTTGAATTTCGATGTGTCAAGTGTGATGATAAGGAAT
ATGGTATTGTTTATTATGAAAAGGACGGTGATGAATCATCTCCAATTTTA
ACAAGTTTTGAATTAGTGAAAGTTCCTGACCCCCAGATGTCTATGGAGAA
TTTAGTTGAGAGCAAACACCACAAGCTTGCCCGGAGTTTAAGAAGTGGAC
CTTCTGACCACGATCTGAAACCCAATGCTGCCACGAGAGATCAGTTAAAT
ATTATTGTGAGTTATCCACCAACCAAGCAACTTACATATGAAGAACAAGA
TCTTGTTTGGAAGTTTAGATATTATCTTACGAATCAAGAAAAAGCCTTGA
CAAAATTCTTGAGATGTGTTAATTGGGATCTACCTCAAGAGGCCAAACAG
GCCTTGGAACTTCTGGGAAAATGGAAGCCGATGGATGTAGAGGACTCCTT
GGAGCTGTTATCCTCTCATTACACCAACCCAACTGTGAGGCGTTATGCTG
TTGCCCGGTTGCGACAGGCCGATGATGAGGATTTGTTGATGTACCTATTA
CAATTGGTCCAGGCTCTCAAATATGAAAATTTTGATGATATAAAGAATGG
ATTGGAACCTACCAAGAAGGATAGTCAGAGTTCAGTGTCAGAAAATGTGT
CAAATTCTGGAATAAATTCTGCAGAAATAGATAGCTCCCAAATTATAACC
AGCCCCCTTCCTTCAGTCTCTTCACCTCCTCCTGCATCAAAAACAAAAGA
AGTTCCAGATGGCGAAAATCTGGAACAAGATCTCTGTACCTTCTTGATAT
CGAGAGCCTGCAAAAACTCAACACTGGCTAATTATTTATACTGGTATGTG
ATAGTGGAATGTGAAGATCAAGATACTCAGCAGAGAGATCCAAAGACCCA
TGAGATGTACTTGAACGTAATGAGAAGATTCAGCCAAGCATTGTTGAAGG
GTGATAAGTCTGTCAGAGTTATGCGTTCTTTGCTGGCTGCACAACAGACA
TTTGTAGATCGGTTGGTGCATCTAATGAAGGCAGTACAACGCGAAAGTGG
AAATCGTAAGAAAAAGAATGAGAGACTACAGGCATTGCTTGGAGATAATG
AAAAGATGAATTTGTCAGATGTGGAACTTATCCCGTTGCCTTTAGAACCC
CAAGTGAAAATTAGAGGAATAATTCCGGAAACAGCTACACTGTTTAAAAG
TGCCCTTATGCCTGCACAGTTGTTTTTTAAGACGGAAGATGGAGGCAAAT
ATCCAGTTATATTTAAGCATGGAGATGATTTACGTCAAGATCAACTTATT
CTTCAAATCATTTCACTCATGGACAAGCTGTTACGGAAAGAAAATCTGGA
CTTGAAATTGACACCTTATAAGGTGTTAGCCACCAGTACAAAACATGGCT
TCATGCAGTTTATCCAGTCAGTTCCTGTGGCTGAAGTTCTTGATACAGAG
GGAAGCATTCAGAACTTTTTTAGAAAATATGCACCAAGTGAGAATGGGCC
AAATGGGATTAGTGCTGAGGTCATGGACACTTACGTTAAAAGCTGTGCTG
GATATTGCGTGATCACCTATATACTTGGAGTTGGAGACAGGCACCTGGAT
AACCTTTTGCTAACAAAAACAGGCAAACTCTTCCACATAGCCTTTGGATA
TATTTTGGGTCGGGATCCAAAGCCTCTTCCTCCACCAATGAAGCTGAATA
AAGAAATGGTAGAAGGAATGGGGGGCACACAGAGTGAGCAGTACCAAGAG
TTCCGTAAACAGTGTTACACGGCTTTCCTCCACCTGCGAAGGTATTCTAA
TCTGATTTTGAACTTGTTTTCCTTGATGGTTGATGCAAACATTCCAGATA
TTGCACTTGAACCAGATAAAACTGTGAAAAAGGTTCAGGATAAATTCCGC
TTAGACCTGTCGGATGAAGAGGCTGTGCATTACATGCAGAGTCTGATTGA
TGAGAGTGTCCATGCTCTTTTTGCTGCAGTGGTGGAACAGATTCACAAGT
TTGCCCAGTACTGGAGAAAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMGEAEKFH
YIYSCDLDINVQLKIGSLGGKREQKSYKAVLEDPMLKFSGLYQETCSDLY
VTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVAL
TIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTK
TPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESE
KRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDP
QMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQL
TYEEQDLVWKFRYYLTNQEKALTKFLRCVNWDLPQEAKQALELLGKWKPM
DVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENF
DDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPP
ASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQ
RDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKA
VQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPET
ATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLL
RKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYA
PSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLF
HIAFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLH
LRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHY
MQSLIDESVHALFAAVVEQIHKFAQYWRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU12350
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression.; ; Estimated MW of expressed protein : 129261 Daltons