cDNA Clone BAP1

• Plasmid: pEBG6P-BAP1
• Parent Plasmid: pEBG6P
• Expressed: GST BAP1
• Genbank: AAH01596.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTT
CACCCTGCTCGTGGAAGATTTCGGTGTCAAGGGGGTGCAAGTGGAGGAGA
TCTACGACCTTCAGAGCAAATGTCAGGGCCCTGTATATGGATTTATCTTC
CTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGTCTCTACCTT
GGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTCT
TTGCCCACCAGCTGATACCCAACTCTTGTGCAACTCATGCCTTGCTGAGC
GTGCTCCTGAACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCAT
GAAGGACTTCACCAAGGGTTTTAGCCCTGAGAGCAAAGGATATGCGATTG
GCAATGCCCCGGAGTTGGCCAAGGCCCATAATAGCCATGCCAGGCCCGAG
CCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGTGCGGACCAT
GGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTG
AGCTGGATGGGCTGAAGGTCTACCCCATTGACCATGGGCCCTGGGGGGAG
GACGAGGAGTGGACAGACAAGGCCCGGCGGGTCATCATGGAGCGTATCGG
CCTCGCCACTGCAGGGGAGCCCTACCACGACATCCGCTTCAACCTGATGG
CAGTGGTGCCCGACCGCAGGATCAAGTATGAGGCCAGGCTGCATGTGCTG
AAGGTGAACCGTCAGACAGTACTAGAGGCTCTGCAGCAGCTGATAAGAGT
AACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAGCTGC
CTGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCA
AACAGGGCCCCTGCAGCCTCTGAGGGCAACCACACAGATGGTGCAGAGGA
GGCGGCTGGTTCATGCGCACAAGCCCCATCCCACAGCCCTCCCAACAAAC
CCAAGCTAGTGGTGAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCC
AACCCCACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGACAATCACAA
TTATGCCAAGTCCCCCATGCAGGAGGAAGAAGACCTGGCGGCAGGTGTGG
GCCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGATGAG
GATGACTATGAGGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGC
CCTTAGGTATAAGGGGAAGGGAACAGGGAAGCCAGGGGCATTGAGCGGTT
CTGCTGATGGGCAACTGTCAGTGCTGCAGCCCAACACCATCAACGTCTTG
GCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCCTCTGTCCAT
CAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACT
CGCAGCCCTCACCCACCCCCAGCAATGAGAGTACAGACACGGCCTCTGAG
ATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGCCAA
CCCGACGCGGCCCTCCAGCCCTGTCACCTCCCACATCTCCAAGGTGCTTT
TTGGAGAGGATGACAGCCTGCTGCGTGTTGACTGCATACGCTACAACCGT
GCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGC
TGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTT
CCTCGCCCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCA
GTGGAGAAGGAGGTCGTGGAAGCCACGGACAGCAGAGAGAAGACGGGGAT
GGTGAGGCCTGGCGAGCCCTTGAGTGGGGAGAAATACTCACCCAAGGAGC
TGCTGGCACTGCTGAAGTGTGTGGAGGCTGAGATTGCAAACTATGAGGCG
TGCCTCAAGGAGGAGGTAGAGAAGAGGAAGAAGTTCAAGATTGATGACCA
GAGAAGGACCCACAACTACGATGAGTTCATCTGCACCTTTATCTCCATGC
TGGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTG
CGGCGGCGCCAAGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCC
TGACCGGCGGAAACGCTCTCGCCCCTACAAGGCCAAGCGCCAGTGAGCGG
CCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNKGWLELESDPG
LFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVS
TLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLS
RMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVR
TMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMER
IGLATAGEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLI
RVTQPELIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGA
EEAAGSCAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDN
HNYAKSPMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTN
SALRYKGKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPL
SIKTSSGAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRS
ANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLH
LAEDGVLSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKT
GMVRPGEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKID
DQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQR
KPDRRKRSRPYKAKRQ

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12770
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 107776 Daltons