cDNA Clone USP20

• Plasmid: pEBG6P-USP20
• Parent Plasmid: pEBG6P
• Expressed: GST USP20
• Genbank: AAH39593.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGG
AGAGGTGACCAAAGAGGACTTGCTGCTCAAATCTAAGGGAACCTGTCAGT
CGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGCCTGC
CCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGC
ACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGACTGT
GGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCC
CCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACC
CTCCCACCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGT
CTGAGTCAGAGGATGATGACCTGAAACCTCGAGGCCTCACGGGCATGAAG
AACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGTCCAA
TTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCA
CAGATAAGAAGCCAGCCCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAG
GTCTGGCATAAGAAACGGCCAAGCTACGTGGTCCCCACCAGTCTGTCTCA
TGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAGGACA
CCCAAGAGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAG
GAGCCGGTGGTGGCCACGGTGGCGCTGACGGAGGCTCGGGACTCAGATTC
GAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAAGATG
AGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAG
GGGCGTGGCGGGGGCAGCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGA
TGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGATGAAGGACCGCAAGT
TCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCT
GATGTGGACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCC
CCCGTCACCACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCGGACAATG
ATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCACCACCAC
GAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAG
GATGGCACCGTCGTACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCA
GCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCATCTCAGACATCTTT
GACGGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATC
CACCACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGG
AGGACCTGGCCAAGCTCCATTCAGCCATCTACCAGAATGTGCCGGCCAAG
CCAGGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGGCCTTCAT
TGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTT
GGGGGCCTGTCGTCACCCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCT
GATGAGTTAAAGGGTGACAACATGTACAGCTGTGAGCGGTGTAAGAAGCT
GCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGT
GCATTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATC
AACAGCCACGTCTCCTTCCCCCTCGAGGGGCTCGACCTGCGCCCCTTCCT
TGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTCTCGGTCA
TCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAG
AACGTGATCAATGGGCAGTGGTACGAGTTTGATGACCAGTACGTCACAGA
AGTCCACGAGACGGTGGTGCAGAACGCCGAGGGCTACGTACTCTTCTACA
GGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTG
GCCGCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTG
GCTCAACAAGTTCAACACCTTCGCGGAGCCAGGCCCCATCACCAACCAGA
CCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTACATC
GACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAA
CAGATTCGGGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGCTCCATCT
GCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGAGGATCGAGATCGAC
ACCTTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGT
CATCTACTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCA
AGGGGAAGGACAACGAGCCCCCCGGGCCCATTGACAACAGCAGGATTGCA
CAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACTACGG
GCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTG
GCCCCGAGATTGCCATCCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAG
AACCTGCACGGGGAGCAGAAGATCGAAGCCGAGACGCGGGCCGTGTGAGC
GGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMGDSRDLCPHLDS
IGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTI
HAQAKKHNLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSP
PPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQAL
SNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVPTSL
SHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDS
DSSDTDEKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETELLI
PDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMAALDQPAEA
QPPSPRSSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPRASP
VRMAPSYVLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDR
VSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYAAQGWLA
FIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCK
KLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRP
FLAKECTSQITTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYV
TEVHETVVQNAEGYVLFYRKSSEEAMRERQQVVSLAAMREPSLLRFYVSR
EWLNKFNTFAEPGPITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVWEHL
YNRFGGGPAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESP
GVIYCISMQWFREWEAFVKGKDNEPPGPIDNSRIAQVKGSGHVQLKQGAD
YGQISEETWTYLNSLYGGGPEIAIRQSVAQPLGPENLHGEQKIEAETRAV
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12776
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression. ; ; Estimated MW of expressed protein : 129301 Daltons