cDNA Clone UCHL5

• Plasmid: pGEX6P1 UCHL5
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C UCHL5
• Genbank: AAH15521.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACGGGCAATGCCGGGGAGTGGTGCCTCATGGAAAGCGACCC
CGGGGTCTTCACCGAGCTCATTAAAGGATTCGGTTGCCGAGGAGCCCAAG
TAGAAGAAATATGGAGTTTAGAGCCTGAGAATTTTGAAAAATTAAAGCCA
GTTCATGGGTTAATTTTTCTTTTCAAGTGGCAGCCAGGAGAAGAACCAGC
AGGCTCTGTGGTTCAGGACTCCCGACTTGACACGATATTTTTTGCTAAGC
AGGTAATTAATAATGCTTGTGCTACTCAAGCCATAGTGAGTGTGTTACTG
AACTGTACCCACCAGGATGTCCATTTAGGCGAGACATTATCAGAGTTTAA
AGAATTTTCACAAAGTTTTGATGCAGCTATGAAAGGCTTGGCACTGAGCA
ATTCAGATGTGATTCGACAAGTACACAACAGTTTCGCCAGACAGCAAATG
TTTGAATTTGATACGAAGACATCAGCAAAAGAAGAAGATGCTTTTCACTT
TGTCAGTTATGTTCCTGTTAATGGGAGACTGTATGAATTAGATGGATTAA
GAGAAGGACCGATTGATTTAGGTGCATGCAATCAAGATGATTGGATCAGT
GCAGTAAGGCCTGTCATAGAAAAAAGGATACAAAAGTACAGTGAAGGTGA
AATTCGATTTAATTTAATGGCCATTGTGTCTGACAGAAAAATGATATATG
AGCAGAAGATAGCAGAGTTACAAAGACAACTTGCAGAGGAACCCATGGAT
ACAGATCAAGGTAATAGTATGTTAAGTGCTATTCAGTCAGAAGTTGCCAA
AAATCAGATGCTTATTGAAGAAGAAGTACAGAAATTAAAAAGATACAAGA
TTGAGAATATCAGAAGGAAGCATAATTATCTGCCTTTCATTATGGAATTG
TTAAAGACTTTAGCAGAACACCAGCAGTTAATACCACTAGTAGAAAAGGC
AAAAGAAAAACAGAACGCAAAGAAAGCTCAGGAAACCAAATGAGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTGNAGEWCLMESDPGVFT
ELIKGFGCRGAQVEEIWSLEPENFEKLKPVHGLIFLFKWQPGEEPAGSVV
QDSRLDTIFFAKQVINNACATQAIVSVLLNCTHQDVHLGETLSEFKEFSQ
SFDAAMKGLALSNSDVIRQVHNSFARQQMFEFDTKTSAKEEDAFHFVSYV
PVNGRLYELDGLREGPIDLGACNQDDWISAVRPVIEKRIQKYSEGEIRFN
LMAIVSDRKMIYEQKIAELQRQLAEEPMDTDQGNSMLSAIQSEVAKNQML
IEEEVQKLKRYKIENIRRKHNYLPFIMELLKTLAEHQQLIPLVEKAKEKQ
NAKKAQETK*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 UCHL5142.38 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12810
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 64300 Daltons
• Low Temp: No