cDNA Clone VPS34

• Plasmid: pEBG6P-VPS34 S282A
• Parent Plasmid: pEBG6P
• Expressed: GST-VPS34
• Genbank: NP_002638
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGGGAAGCAGAGAAGTTTCACTACATCTATAGTTGTGA
CCTGGATATCAACGTCCAGCTTAAGATAGGAAGCTTGGGAGGGAAGAGAG
AACAAAAGAGTTATAAAGCTGTCCTGGAAGACCCAATGTTGAAGTTCTCA
GGACTATATCAAGAGACATGCTCTGATCTTTATGTTACTTGTCAAGTTTT
TGCAGAAGGGAAGCCTTTGGCCTTGCCAGTGAGAACATCCTACAAAGCAT
TTAGTACAAGATGGAACTGGAATGAATGGCTGAAACTACCAGTAAAATAC
CCTGACCTGCCCAGGAATGCCCAAGTGGCCCTCACCATATGGGATGTGTA
TGGTCCCGGAAAAGCAGTGCCTGTAGGAGGAACAACGGTTTCGCTCTTTG
GAAAATACGGCATGTTTCGCCAAGGGATGCATGACTTGAAAGTCTGGCCT
AATGTAGAAGCAGATGGATCAGAACCCACAAAAACTCCTGGCAGAACAAG
TAGCACTCTCTCAGAAGATCAGATGAGCCGTCTTGCCAAGCTCACCAAAG
CTCATCGACAAGGACACATGGTGAAAGTAGATTGGCTGGATAGATTGACA
TTTAGAGAAATAGAAATGATAAATGAGAGTGAAAAACGAAGTTCTAATTT
CATGTACCTGATGGTTGAATTTCGATGTGTCAAGTGTGATGATAAGGAAT
ATGGTATTGTTTATTATGAAAAGGACGGTGATGAATCATCTCCAATTTTA
ACAAGTTTTGAATTAGTGAAAGTTCCTGACCCCCAGATGTCTATGGAGAA
TTTAGTTGAGAGCAAACACCACAAGCTTGCCCGGAGTTTAAGAAGTGGAC
CTGCTGACCACGATCTGAAACCCAATGCTGCCACGAGAGATCAGTTAAAT
ATTATTGTGAGTTATCCACCAACCAAGCAACTTACATATGAAGAACAAGA
TCTTGTTTGGAAGTTTAGATATTATCTTACGAATCAAGAAAAAGCCTTGA
CAAAATTCTTGAGATGTGTTAATTGGGATCTACCTCAAGAGGCCAAACAG
GCCTTGGAACTTCTGGGAAAATGGAAGCCGATGGATGTAGAGGACTCCTT
GGAGCTGTTATCCTCTCATTACACCAACCCAACTGTGAGGCGTTATGCTG
TTGCCCGGTTGCGACAGGCCGATGATGAGGATTTGTTGATGTACCTATTA
CAATTGGTCCAGGCTCTCAAATATGAAAATTTTGATGATATAAAGAATGG
ATTGGAACCTACCAAGAAGGATAGTCAGAGTTCAGTGTCAGAAAATGTGT
CAAATTCTGGAATAAATTCTGCAGAAATAGATAGCTCCCAAATTATAACC
AGCCCCCTTCCTTCAGTCTCTTCACCTCCTCCTGCATCAAAAACAAAAGA
AGTTCCAGATGGCGAAAATCTGGAACAAGATCTCTGTACCTTCTTGATAT
CGAGAGCCTGCAAAAACTCAACACTGGCTAATTATTTATACTGGTATGTG
ATAGTGGAATGTGAAGATCAAGATACTCAGCAGAGAGATCCAAAGACCCA
TGAGATGTACTTGAACGTAATGAGAAGATTCAGCCAAGCATTGTTGAAGG
GTGATAAGTCTGTCAGAGTTATGCGTTCTTTGCTGGCTGCACAACAGACA
TTTGTAGATCGGTTGGTGCATCTAATGAAGGCAGTACAACGCGAAAGTGG
AAATCGTAAGAAAAAGAATGAGAGACTACAGGCATTGCTTGGAGATAATG
AAAAGATGAATTTGTCAGATGTGGAACTTATCCCGTTGCCTTTAGAACCC
CAAGTGAAAATTAGAGGAATAATTCCGGAAACAGCTACACTGTTTAAAAG
TGCCCTTATGCCTGCACAGTTGTTTTTTAAGACGGAAGATGGAGGCAAAT
ATCCAGTTATATTTAAGCATGGAGATGATTTACGTCAAGATCAACTTATT
CTTCAAATCATTTCACTCATGGACAAGCTGTTACGGAAAGAAAATCTGGA
CTTGAAATTGACACCTTATAAGGTGTTAGCCACCAGTACAAAACATGGCT
TCATGCAGTTTATCCAGTCAGTTCCTGTGGCTGAAGTTCTTGATACAGAG
GGAAGCATTCAGAACTTTTTTAGAAAATATGCACCAAGTGAGAATGGGCC
AAATGGGATTAGTGCTGAGGTCATGGACACTTACGTTAAAAGCTGTGCTG
GATATTGCGTGATCACCTATATACTTGGAGTTGGAGACAGGCACCTGGAT
AACCTTTTGCTAACAAAAACAGGCAAACTCTTCCACATAGACTTTGGATA
TATTTTGGGTCGGGATCCAAAGCCTCTTCCTCCACCAATGAAGCTGAATA
AAGAAATGGTAGAAGGAATGGGGGGCACACAGAGTGAGCAGTACCAAGAG
TTCCGTAAACAGTGTTACACGGCTTTCCTCCACCTGCGAAGGTATTCTAA
TCTGATTTTGAACTTGTTTTCCTTGATGGTTGATGCAAACATTCCAGATA
TTGCACTTGAACCAGATAAAACTGTGAAAAAGGTTCAGGATAAATTCCGC
TTAGACCTGTCGGATGAAGAGGCTGTGCATTACATGCAGAGTCTGATTGA
TGAGAGTGTCCATGCTCTTTTTGCTGCAGTGGTGGAACAGATTCACAAGT
TTGCCCAGTACTGGAGAAAATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMGEA
EKFHYIYSCDLDINVQLKIGSLGGKREQKSYKAVLEDPMLKFSGLYQETC
SDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNA
QVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGS
EPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMI
NESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVK
VPDPQMSMENLVESKHHKLARSLRSGPADHDLKPNAATRDQLNIIVSYPP
TKQLTYEEQDLVWKFRYYLTNQEKALTKFLRCVNWDLPQEAKQALELLGK
WKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALK
YENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVS
SPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQ
DTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVH
LMKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGI
IPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLM
DKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFF
RKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKT
GKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYT
AFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEE
AVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU12904
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for mammalian expression. Differs to genbank at E27G K335R.; ; Estimated MW of expressed protein : 129691 Daltons