cDNA Clone UCHL1

• Plasmid: pEBG6P-UCHL1
• Parent Plasmid: pEBG6P
• Expressed: GST UCHL1
• Genbank: AAH00332.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCAGCTCAAGCCGATGGAGATCAACCCCGAGATGCTGAACAA
AGTGCTGTCCCGGCTGGGGGTCGCCGGCCAGTGGCGCTTCGTGGACGTGC
TGGGGCTGGAAGAGGAGTCTCTGGGCTCGGTGCCAGCGCCTGCCTGCGCG
CTGCTGCTGCTGTTTCCCCTCACGGCCCAGCATGAGAACTTCAGGAAAAA
GCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTCCTAAAGTGTACTTCA
TGAAGCAGACCATTGGGAATTCCTGTGGCACAATCGGACTTATTCACGCA
GTGGCCAATAATCAAGACAAACTGGGATTTGAGGATGGATCAGTTCTGAA
ACAGTTTCTTTCTGAAACAGAGAAAATGTCCCCTGAAGACAGAGCAAAAT
GCTTTGAAAAGAATGAGGCCATACAGGCAGCCCATGATGCCGTGGCACAG
GAAGGCCAATGTCGGGTAGATGACAAGGTGAATTTCCATTTTATTCTGTT
TAACAACGTGGATGGCCACCTCTATGAACTTGATGGACGAATGCCTTTTC
CGGTGAACCATGGCGCCAGTTCAGAGGACACCCTGCTGAAGGACGCTGCC
AAGGTCTGCAGAGAATTCACCGAGCGTGAGCAAGGAGAAGTCCGCTTCTC
TGCCGTGGCTCTCTGCAAGGCAGCCTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMQLKPMEINPEML
NKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFR
KKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSV
LKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFI
LFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVR
FSAVALCKAA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU15085
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 52240 Daltons