cDNA Clone WDR48

• Synonyms: P80, SPG60, UAF1
• Plasmid: pGEX6P2 WDR48
• Parent Plasmid: pGEX6P2
• Expressed: GST 3C WDR48
• Genbank: NP_065890.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGCCCATCACCGGCAGAACACAGCAGGGCGGAGGAA
AGTGCAGGTTTCCTATGTTATTCGAGATGAAGTGGAGAAGTACAACCGAA
ATGGAGTCAATGCTCTGCAGCTGGATCCAGCACTAAATAGACTTTTCACA
GCCGGTCGAGACTCTATCATAAGAATATGGAGTGTCAATCAGCACAAGCA
AGATCCATATATAGCATCTATGGAACACCATACTGATTGGGTAAACGACA
TTGTACTCTGTTGTAATGGGAAAACATTAATATCTGCTTCTTCTGACACG
ACAGTAAAAGTATGGAATGCACACAAGGGATTTTGCATGTCAACATTAAG
GACACATAAGGATTACGTAAAGGCCTTAGCATATGCCAAGGATAAAGAAC
TAGTAGCATCAGCTGGGTTGGACAGACAAATATTCCTTTGGGATGTGAAT
ACTCTAACAGCATTGACTGCCTCAAATAACACTGTCACAACTTCTTCTTT
AAGTGGAAACAAAGATTCCATTTATAGCCTGGCCATGAATCAACTGGGAA
CAATCATTGTATCAGGGTCCACTGAAAAGGTGTTACGGGTATGGGATCCA
AGAACATGTGCAAAACTAATGAAGCTTAAAGGGCACACGGATAATGTGAA
GGCATTGCTATTAAACAGAGATGGCACGCAATGCCTGTCAGGCAGTTCTG
ATGGGACAATTCGCCTTTGGTCCCTTGGCCAGCAGAGATGTATAGCAACA
TACCGAGTCCATGATGAAGGTGTTTGGGCGCTGCAAGTCAATGATGCCTT
CACACATGTGTATTCTGGTGGAAGGGACAGGAAGATTTATTGTACAGACC
TAAGAAACCCTGACATTCGGGTGCTAATTTGTGAAGAAAAAGCACCAGTT
CTCAAGATGGAGCTTGATAGATCAGCTGATCCTCCTCCTGCAATTTGGGT
TGCAACAACTAAGTCTACAGTAAATAAATGGACTTTGAAAGGAATTCATA
ATTTTAGAGCCTCTGGAGATTATGACAATGACTGTACAAATCCTATAACA
CCTCTTTGTACACAACCTGACCAGGTTATTAAAGGGGGTGCTAGTATTAT
TCAGTGCCACATTCTTAATGATAAGAGACATATATTAACCAAAGATACCA
ATAATAATGTGGCATATTGGGATGTATTGAAGGCATGTAAAGTTGAAGAT
CTGGGCAAAGTGGATTTTGAAGATGAAATTAAGAAAAGATTTAAAATGGT
GTATGTGCCAAATTGGTTCTCAGTAGACTTAAAAACAGGGATGTTAACTA
TTACTTTGGATGAAAGTGATTGTTTTGCTGCCTGGGTTTCTGCAAAAGAT
GCTGGTTTCAGCAGCCCTGATGGGTCAGATCCAAAATTGAATTTAGGAGG
ACTTTTACTCCAAGCACTCCTGGAATATTGGCCTAGAACACATGTGAATC
CAATGGATGAAGAGGAAAATGAAGTAAACCATGTAAATGGGGAGCAGGAG
AACCGAGTGCAGAAGGGAAATGGATATTTTCAAGTGCCCCCACATACACC
CGTGATCTTTGGTGAAGCTGGAGGTCGCACACTGTTCAGGCTGCTCTGCC
GAGATTCCGGGGGTGAGACTGAGTCTATGCTTCTTAATGAAACAGTGCCA
CAATGGGTAATTGACATCACTGTGGATAAAAATATGCCCAAATTCAACAA
AATTCCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCAAAAACCTTAA
AAAAAGATAGACTCTCTGCTAGTGACATGCTCCAAGTCCGAAAAGTTATG
GAACATGTTTATGAAAAAATTATCAACTTGGATAATGAGTCTCAAACCAC
TAGCTCTTCTAATAATGAAAAACCAGGAGAACAGGAAAAAGAAGAAGATA
TTGCTGTGTTGGCAGAGGAGAAAATTGAACTTTTGTGCCAAGACCAGGTT
TTGGATCCAAATATGGACCTTCGAACAGTGAAACACTTCATATGGAAGAG
CGGTGGAGACCTCACCCTCCATTACCGTCAGAAGTCCACGTGAGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMAAHHRQN
TAGRRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWS
VNQHKQDPYIASMEHHTDWVNDIVLCCNGKTLISASSDTTVKVWNAHKGF
CMSTLRTHKDYVKALAYAKDKELVASAGLDRQIFLWDVNTLTALTASNNT
VTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKG
HTDNVKALLLNRDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWAL
QVNDAFTHVYSGGRDRKIYCTDLRNPDIRVLICEEKAPVLKMELDRSADP
PPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLCTQPDQVIK
GGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIK
KRFKMVYVPNWFSVDLKTGMLTITLDESDCFAAWVSAKDAGFSSPDGSDP
KLNLGGLLLQALLEYWPRTHVNPMDEEENEVNHVNGEQENRVQKGNGYFQ
VPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKN
MPKFNKIPFYLQPHASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLD
NESQTTSSSNNEKPGEQEKEEDIAVLAEEKIELLCQDQVLDPNMDLRTVK
HFIWKSGGDLTLHYRQKST*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P2 WDR4859.86 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU15376
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 104010 Daltons
• Low Temp: No