cDNA Clone TTK

• Plasmid: pMalP TTK
• Parent Plasmid: pMAL6P
• Expressed: MBP TTK
• Genbank: AAH00633.1
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGATTC
CATAATGAACAAAGTGAGAGACATTAAAAATAAGTTTAAAAATGAAGACC
TTACTGATGAACTAAGCTTGAATAAAATTTCTGCTGATACTACAGATAAC
TCGGGAACTGTTAACCAAATTATGATGATGGCAAACAACCCAGAGGACTG
GTTGAGTTTGTTGCTCAAACTAGAGAAAAACAGTGTTCCGCTAAGTGATG
CTCTTTTAAATAAATTGATTGGTCGTTACAGTCAAGCAATTGAAGCGCTT
CCCCCAGATAAATATGGCCAAAATGAGAGTTTTGCTAGAATTCAAGTGAG
ATTTGCTGAATTAAAAGCTATTCAAGAGCCAGATGATGCACGTGACTACT
TTCAAATGGCCAGAGCAAACTGCAAGAAATTTGCTTTTGTTCATATATCT
TTTGCACAATTTGAACTGTCACAAGGTAATGTCAAAAAAAGTAAACAACT
TCTTCAAAAAGCTGTAGAACGTGGAGCAGTACCACTAGAAATGCTGGAAA
TTGCCCTGCGGAATTTAAACCTCCAAAAAAAGCAGCTGCTTTCAGAGGAG
GAAAAGAAGAATTTATCAGCATCTACGGTATTAACTGCCCAAGAATCATT
TTCCGGTTCACTTGGGCATTTACAGAATAGGAACAACAGTTGTGATTCCA
GAGGACAGACTACTAAAGCCAGGTTTTTATATGGAGAGAACATGCCACCA
CAAGATGCAGAAATAGGTTACCGGAATTCATTGAGACAAACTAACAAAAC
TAAACAGTCATGCCCATTTGGAAGAGTCCCAGTTAACCTTCTAAATAGCC
CAGATTGTGATGTGAAGACAGATGATTCAGTTGTACCTTGTTTTATGAAA
AGACAAACCTCTAGATCAGAATGCCGAGATTTGGTTGTGCCTGGATCTAA
ACCAAGTGGAAATGATTCCTGTGAATTAAGAAATTTAAAGTCTGTTCAAA
ATAGTCATTTCAAGGAACCTCTGGTGTCAGATGAAAAGAGTTCTGAACTT
ATTATTACTGATTCAATAACCCTGAAGAATAAAACGGAATCAAGTCTTCT
AGCTAAATTAGAAGAAACTAAAGAGTATCAAGAACCAGAGGTTCCAGAGA
GTAACCAGAAACAGTGGCAATCTAAGAGAAAGTCAGAGTGTATTAACCAG
AATCCTGCTGCATCTTCAAATCACTGGCAGATTCCGGAGTTAGCCCGAAA
AGTTAATACAGAGCAGAAACATACCACTTTTGAGCAACCTGTCTTTTCAG
TTTCAAAACAGTCACCACCAATATCAACATCTAAATGGTTTGACCCAAAA
TCTATTTGTAAGACACCAAGCAGCAATACCTTGGATGATTACATGAGCTG
TTTTAGAACTCCAGTTGTAAAGAATGACTTTCCACCTGCTTGTCAGTTGT
CAACACCTTATGGCCAACCTGCCTGTTTCCAGCAGCAACAGCATCAAATA
CTTGCCACTCCACTTCAAAATTTACAGGTTTTAGCATCTTCTTCAGCAAA
TGAATGCATTTCGGTTAAAGGAAGAATTTATTCCATTTTAAAGCAGATAG
GAAGTGGAGGTTCAAGCAAGGTATTTCAGGTGTTAAATGAAAAGAAACAG
ATATATGCTATAAAATATGTGAACTTAGAAGAAGCAGATAACCAAACTCT
TGATAGTTACCGGAACGAAATAGCTTATTTGAATAAACTACAACAACACA
GTGATAAGATCATCCGACTTTATGATTATGAAATCACGGACCAGTACATC
TACATGGTAATGGAGTGTGGAAATATTGATCTTAATAGTTGGCTTAAAAA
GAAAAAATCCATTGATCCATGGGAACGCAAGAGTTACTGGAAAAATATGT
TAGAGGCAGTTCACACAATCCATCAACATGGCATTGTTCACAGTGATCTT
AAACCAGCTAACTTTCTGATAGTTGATGGAATGCTAAAGCTAATTGATTT
TGGGATTGCAAACCAAATGCAACCAGATACAACAAGTGTTGTTAAAGATT
CTCAGGTTGGCACAGTTAATTATATGCCACCAGAAGCAATCAAAGATATG
TCTTCCTCCAGAGAGAATGGGAAATCTAAGTCAAAGATAAGCCCCAAAAG
TGATGTTTGGTCCTTAGGATGTATTTTGTACTATATGACTTACGGGAAAA
CACCATTTCAGCAGATAATTAATCAGATTTCTAAATTACATGCCATAATT
GATCCTAATCATGAAATTGAATTTCCCGATATTCCAGAGAAAGATCTTCA
AGATGTGTTAAAGTGTTGTTTAAAAAGGGACCCAAAACAGAGGATATCCA
TTCCTGAGCTCCTGGCTCATCCCTATGTTCAAATTCAAACTCATCCAGTT
AACCAAATGGCCAAGGGAACCACTGAAGAAATGAAATATGTTCTGGGCCA
ACTTGTTGGTCTGAATTCTCCTAACTCCATTTTGAAAGCTGCTAAAACTT
TATATGAACACTATAGTGGTGGTGAAAGTCATAATTCTTCATCCTCCAAG
ACTTTTGAAAAAAAAAGGGGAAAAAAATGAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMESEDLSGRELTIDSIMNKVRD
IKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKL
EKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAI
QEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVER
GAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHL
QNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFG
RVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSC
ELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETK
EYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKH
TTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVK
NDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKG
RIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEI
AYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPW
ERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQ
PDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGC
ILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCL
KRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSP
NSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15476
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression.; ; Estimated MW of expressed protein : 143808 Daltons