cDNA Clone UCHL1

• Plasmid: pET156P UCHL1
• Parent Plasmid: pET15b 6His 3C
• Genbank: AAH00332.1
• Species: Human

Sequence of Insert

GGATCCATGCAGCTCAAGCCGATGGAGATCAACCCCGAGATGCTGAACAA
AGTGCTGTCCCGGCTGGGGGTCGCCGGCCAGTGGCGCTTCGTGGACGTGC
TGGGGCTGGAAGAGGAGTCTCTGGGCTCGGTGCCAGCGCCTGCCTGCGCG
CTGCTGCTGCTGTTTCCCCTCACGGCCCAGCATGAGAACTTCAGGAAAAA
GCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTCCTAAAGTGTACTTCA
TGAAGCAGACCATTGGGAATTCCTGTGGCACAATCGGACTTATTCACGCA
GTGGCCAATAATCAAGACAAACTGGGATTTGAGGATGGATCAGTTCTGAA
ACAGTTTCTTTCTGAAACAGAGAAAATGTCCCCTGAAGACAGAGCAAAAT
GCTTTGAAAAGAATGAGGCCATACAGGCAGCCCATGATGCCGTGGCACAG
GAAGGCCAATGTCGGGTAGATGACAAGGTGAATTTCCATTTTATTCTGTT
TAACAACGTGGATGGCCACCTCTATGAACTTGATGGACGAATGCCTTTTC
CGGTGAACCATGGCGCCAGTTCAGAGGACACCCTGCTGAAGGACGCTGCC
AAGGTCTGCAGAGAATTCACCGAGCGTGAGCAAGGAGAAGTCCGCTTCTC
TGCCGTGGCTCTCTGCAAGGCAGCCTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMQLKPMEINPEMLNKVLSRLGVAGQWR
FVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSP
KVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPE
DRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDG
RMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15693
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 27267 Daltons