cDNA Clone Bora

• Plasmid: pGEX6P-2-Bora
• Parent Plasmid: pGEX6P2
• Expressed: GST-Bora
• Genbank: NM_024808.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGAGATGTCAAGGAATCAAAGATGCAAATAACACCAGA
AACTCCAGGAAGGATCCCTGTTTTAAATCCTTTTGAAAGTCCTAGTGATT
ATTCTAATCTCCATGAACAAACTCTCGCCAGTCCTTCTGTTTTTAAATCA
ACAAAATTACCAACTCCAGGGAAATTTAGATGGTCTATTGATCAACTAGC
TGTAATAAATCCTGTAGAAATAGACCCAGAAGATATTCATCGTCAAGCTT
TATACTTAAGTCATTCTCGAATAGATAAAGATGTGGAAGACAAAAGACAA
AAAGCCATTGAAGAGTTTTTCACTAAAGATGTCATCGTACCCTCTCCTTG
GACTGATCATGAAGGGAAACAGCTTTCACAATGTCATTCCAGTAAATGCA
CTAACATAAATAGTGACTCTCCAGTTGGAAAAAAGCTGACCATTCATTCT
GAGAAAAGCGATGCTGCTTGTCAGACATTGCTGTCTCTTCCTGTGGATTT
TAATTTAGAAAATATATTAGGTGACTATTTTAGAGCTGATGAATTTGCAG
ATCAATCTCCTGGAAACCTCAGTTCTTCATCCCTCAGAAGAAAGCTGTTT
TTAGATGGGAACGGAAGCATCTCCGACTCCTTACCTTCGGCTTCTCCCGG
AAGTCCTCACAGTGGTGTTCAAACATCACTAGAGATGTTTTATTCAATAG
ATTTGTCTCCTGTAAAGTGTAGGAGCCCCTTGCAGACACCAAGTTCGGGG
CAGTTTTCTTCTAGCCCTATTCAGGCTAGTGCAAAAAAATACAGCTTGGG
AAGCATAACTAGTCCTTCGCCTATTTCTTCACCCACTTTCTCACCAATTG
AATTTCAGATAGGAGAGACTCCACTCTCAGAACAAAGGAAGTTTACTGTT
CATTCTCCTGATGCTTCATCTGGAACAAATTCTAATGGGATAACTAATCC
GTGTATCAGAAGTCCTTATATAGATGGCTGCTCGCCAATTAAAAATTGGT
CTCCTATGAGACTTCAGATGTATAGTGGTGGTACTCAGTATCGGACCTCA
GTGATTCAGATACCTTTTACTCTTGAGACTCAAGGTGAAGATGAGGAAGA
TAAAGAGAATATTCCTTCCACAGATGTCTCATCACCCGCCATGGATGCTG
CTGGAATACACCTACGGCAGTTTAGTAATGAGGCTTCTACCCATGGTACA
CATTTGGTTGTGACTGCCATGTCTGTTACACAAAATCAGTCCAGTGCTTC
TGAGAAAGAATTAGCACTGTTGCAGGATGTTGAAAGGGAGAAAGACAATA
ACACTGTGGATATGGTTGATCCTATAGAGATAGCAGATGAGACCACTTGG
ATTAAGGAGCCGGTTGATAATGGCAGTTTACCCATGACTGATTTTGTAAG
TGGCATTGCCTTCAGTATTGAAAACTCTCATATGTGCATGTCACCTCTTG
CTGAAAGCAGTGTCATTCCTTGTGAAAGCAGTAACATTCAGATGGATAGT
GGCTATAATACGCAGAATTGTGGAAGCAATATTATGGATACAGTTGGGGC
AGAAAGTTACTGCAAAGAAAGTGATGCACAAACATGTGAAGTTGAGAGTA
AATCTCAAGCATTTAATATGAAGCAAGACCACACAACACAGAGGTGTTGG
ATGAAAACAGCAAGCCCTTTTCAATGCAGCAGTCCATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMGDVKESK
MQITPETPGRIPVLNPFESPSDYSNLHEQTLASPSVFKSTKLPTPGKFRW
SIDQLAVINPVEIDPEDIHRQALYLSHSRIDKDVEDKRQKAIEEFFTKDV
IVPSPWTDHEGKQLSQCHSSKCTNINSDSPVGKKLTIHSEKSDAACQTLL
SLPVDFNLENILGDYFRADEFADQSPGNLSSSSLRRKLFLDGNGSISDSL
PSASPGSPHSGVQTSLEMFYSIDLSPVKCRSPLQTPSSGQFSSSPIQASA
KKYSLGSITSPSPISSPTFSPIEFQIGETPLSEQRKFTVHSPDASSGTNS
NGITNPCIRSPYIDGCSPIKNWSPMRLQMYSGGTQYRTSVIQIPFTLETQ
GEDEEDKENIPSTDVSSPAMDAAGIHLRQFSNEASTHGTHLVVTAMSVTQ
NQSSASEKELALLQDVEREKDNNTVDMVDPIEIADETTWIKEPVDNGSLP
MTDFVSGIAFSIENSHMCMSPLAESSVIPCESSNIQMDSGYNTQNCGSNI
MDTVGAESYCKESDAQTCEVESKSQAFNMKQDHTTQRCWMKTASPFQCSS
P*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU15717
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression.; ; Estimated MW of expressed protein : 89004 Daltons