cDNA Clone UBA7

• Plasmid: pGEX6P-2-UBA7
• Parent Plasmid: pGEX6P1
• Expressed: GST UBA7
• Genbank: NP_003326.2
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCGCGATGGATGCCCTGGACGCTTCGAAGCTACTGGATGAGGAGCTG
TATTCAAGACAGCTGTATGTGCTGGGCTCACCTGCCATGCAGAGGATTCA
GGGAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGTGG
CCAAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCC
CACCCCACCTGCTGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAGCA
GGACTTGGAAAGGAGCAGAGCCGAGGCCTCTCAAGAGCTCTTGGCTCAGC
TCAACAGAGCTGTCCAGGTCGTCGTGCACACGGGTGACATCACTGAGGAC
CTGCTGTTGGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAGCTGGAGGA
GCAGCTGAAGGTGGGCACCTTGTGTCATAAGCATGGAGTTTGCTTTCTGG
CGGCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTGAG
GACTTCACTGTGCAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCAT
CCAGCACATCTCCCAGGGCTCCCCTGGCATTCTCACTCTGAGGAAAGGGG
CCAATACCCACTACTTCCGTGATGGAGACTTGGTGACTTTCTCGGGAATT
GAGGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATCCACGTGCG
GGAGGATGGGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACT
TGCGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAGACTGTGAGACAT
AAGTCCCTGGACACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGCTC
CCAGGAAGTTCACCATGCCCACTGCCTGCATCAGGCCTTCTGTGCACTGC
ACAAGTTCCAGCACCTCCATGGCCGGCCACCCCAGCCCTGGGATCCTGTT
GATGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGAACCACTGAAGCG
GACAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGA
CAGTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTG
GGTGCAGTAGCTGCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTCAT
GCCTCTGGACCAGTGGCTTTACTTTGATGCCCTCGATTGTCTTCCGGAAG
ATGGGGAGCTCCTTCCCAGTCCTGAGGACTGTGCCCTGAGAGGCAGCCGC
TATGATGGGCAAATTGCAGTGTTTGGGGCTGGTTTTCAGGAGAAACTGAG
ACGCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTGGTTGTGAGCTGC
TCAAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTG
ACTGTTGTTGACATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTT
CCTCTTCAGGTCCCAGGACGTTGGTAGACCCAAGGCAGAGGTGGCTGCAG
CAGCTGCCCGGGGCCTGAACCCAGACTTACAGGTGATCCCGCTCACCTAC
CCACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTTTTCTCCCG
TGTGGATGGTGTGGCTGCTGCCCTGGACAGTTTCCAGGCCCGGCGCTATG
TGGCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTGGAGGCAGGCACA
TCGGGCACCTGGGGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGC
CTACAGAGCCCCTGCCTCAGCTGCAGCTTCTGAGGATGCCCCCTACCCTG
TCTGTACCGTGCGGTACTTCCCTAGCACAGCCGAGCACACCCTGCAGTGG
GCCCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAGACCATCAA
CCACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGA
CACTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGAGTGCGTCCACAG
AACTGGCAAGACTGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGCTT
TCATTATGGCATCAAACAGCTGCTGAGGCACTTCCCACCTAATAAAGTGC
TTGAGGATGGAACTCCCTTCTGGTCAGGTCCCAAACAGTGTCCCCAGCCC
TTGGAGTTTGACACCAACCAAGACACACACCTCCTCTACGTACTGGCAGC
TGCCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTGGA
CTGCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAG
ATGGCCCCCATCTTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCTGA
GTTTGGCCCTGAGCAGCAGAAGGAACTGAACAAAGCCCTGGAAGTCTGGA
GTGTGGGCCCTCCCCTGAAGCCTCTGATGTTTGAGAAGGATGATGACAGC
AACTTCCATGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGATGTCAGAA
CTACGGGATTCCACCGGTCAACCGTGCCCAGAGCAAGCGAATTGTGGGCC
AGATTATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGTTG
GGCCTGGAGCTGTATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTT
TCGCCACAGCTACCTACATCTGGCTGAAAACTACCTCATCCGCTATATGC
CTTTTGCCCCAGCCATCCAGACGTTCCATCACCTGAAGTGGACCTCTTGG
GACCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTGGAGTCGCT
GCTGGCTCATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGC
ACGGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCTGAAAAGCAGGCC
CAGCACCTGCCCCTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGCCA
GGCACCTGCTCCTGGGCAGCGGGTGTTGGTGCTAGAGCTGAGCTGTGAGG
GTGACGACGAGGACACTGCCTTCCCACCTCTGCACTATGAGCTGTAGGCG
GCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDALDASK
LLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGS
LTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHT
GDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQL
FCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDL
VTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKR
PKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPP
QPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLS
PMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDC
ALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGA
GNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQ
VIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKP
LLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTA
EHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGV
LRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGP
KQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLP
QPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMF
EKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTA
AVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHH
LKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGW
SPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPL
HYEL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32095
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 139409 Daltons