cDNA Clone UBE20

• Plasmid: pGEX6P-1-UBE20
• Parent Plasmid: pGEX6P1
• Expressed: GST UBE20
• Genbank: AAH51868
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATG
CAACATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACG
AGTTCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCA
GACCCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTAC
CTGCATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGC
TGATTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCT
GACTTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGA
GGATGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCC
GTGTGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAG
ACCATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGA
GTCAGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATG
AATGGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAG
GACGAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCC
GGTGGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAG
CTGCCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATG
GAGAAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGA
GGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGC
TGACGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGG
GAGAAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAA
GACCCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGC
CCGACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAG
GCTGAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAA
GCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGG
AGTTTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCA
GAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGC
TACCTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGG
ACCTCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGAT
GGCCTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCC
CCCCCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACC
TGTATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGA
AAGGGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCAT
CTCCATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCG
GCTTCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGT
TACAATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGT
GCGGCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCA
CTGGTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCAT
GCCCTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAG
CAGCTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAAC
AAGAACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCA
GACTCAGAGGGCGGTGCCCAGGGCCTGGCCTCAGCTAGCAGGGACCACAC
AGACCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGA
AACCAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGT
GGCTACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCAT
CAAGAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGG
CAGGCATGCCGGAGTGCACAGAGGACAAGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTSADVMWQDGSVECNIRS
NDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVK
WFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAP
HKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYD
SVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPE
DKGVVISEEAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKI
LESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDN
VAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVT
FTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLP
EGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFC
YLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQG
LILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPP
EVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPE
PPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTS
ETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIR
GVLTQFRAALLEAGMPECTEDK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32144
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed : 108539 Daltons