cDNA Clone UBE20

• Plasmid: pET156P UBE20
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBE20
• Genbank: AAH51868
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATG
CAACATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACG
AGTTCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCA
GACCCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTAC
CTGCATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGC
TGATTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCT
GACTTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGA
GGATGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCC
GTGTGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAG
ACCATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGA
GTCAGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATG
AATGGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAG
GACGAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCC
GGTGGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAG
CTGCCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATG
GAGAAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGA
GGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGC
TGACGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGG
GAGAAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAA
GACCCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGC
CCGACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAG
GCTGAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAA
GCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGG
AGTTTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCA
GAAGCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGC
TACCTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGG
ACCTCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGAT
GGCCTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCC
CCCCCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACC
TGTATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGA
AAGGGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCAT
CTCCATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCG
GCTTCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGT
TACAATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGT
GCGGCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCA
CTGGTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCAT
GCCCTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAG
CAGCTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAAC
AAGAACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCA
GACTCAGAGGGCGGTGCCCAGGGCCTGGCCTCAGCTAGCAGGGACCACAC
AGACCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGA
AACCAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGT
GGCTACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCAT
CAAGAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGG
CAGGCATGCCGGAGTGCACAGAGGACAAGTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMTSADVMWQDGSVECNIRSNDLFPVHH
LDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSG
DDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSV
GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSG
ASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISE
EAATAAVQGAVAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMT
VEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEK
MEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEV
FSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTF
EDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGR
LNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPY
YNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIR
QHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELS
DSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASV
PPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRA
ALLEAGMPECTEDK

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32152
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression; ; Estimated MW of expressed : 84160 Daltons