cDNA Clone BIRC2

• Plasmid: pEBG6P-BIRC2
• Parent Plasmid: pEBG6P
• Expressed: GST BIRC2
• Genbank: NP_001157.1
• Tag: GST

Sequence of Insert

GCGGCCGCGATGCACAAAACTGCCTCCCAAAGACTTTTCCCAGGTCCCTC
GTATCAAAACATTAAGAGTATAATGGAAGATAGCACGATCTTGTCAGATT
GGACAAACAGCAACAAACAAAAAATGAAGTATGACTTTTCCTGTGAACTC
TACAGAATGTCTACATATTCAACTTTCCCCGCCGGGGTGCCTGTCTCAGA
AAGGAGTCTTGCTCGTGCTGGTTTTTATTATACTGGTGTGAATGACAAGG
TCAAATGCTTCTGTTGTGGCCTGATGCTGGATAACTGGAAACTAGGAGAC
AGTCCTATTCAAAAGCATAAACAGCTATATCCTAGCTGTAGCTTTATTCA
GAATCTGGTTTCAGCTAGTCTGGGATCCACCTCTAAGAATACGTCTCCAA
TGAGAAACAGTTTTGCACATTCATTATCTCCCACCTTGGAACATAGTAGC
TTGTTCAGTGGTTCTTACTCCAGCCTTTCTCCAAACCCTCTTAATTCTAG
AGCAGTTGAAGACATCTCTTCATCGAGGACTAACCCCTACAGTTATGCAA
TGAGTACTGAAGAAGCCAGATTTCTTACCTACCATATGTGGCCATTAACT
TTTTTGTCACCATCAGAATTGGCAAGAGCTGGTTTTTATTATATAGGACC
TGGAGATAGGGTAGCCTGCTTTGCCTGTGGTGGGAAGCTCAGTAACTGGG
AACCAAAGGATGATGCTATGTCAGAACACCGGAGGCATTTTCCCAACTGT
CCATTTTTGGAAAATTCTCTAGAAACTCTGAGGTTTAGCATTTCAAATCT
GAGCATGCAGACACATGCAGCTCGAATGAGAACATTTATGTACTGGCCAT
CTAGTGTTCCAGTTCAGCCTGAGCAGCTTGCAAGTGCTGGTTTTTATTAT
GTGGGTCGCAATGATGATGTCAAATGCTTTTGTTGTGATGGTGGCTTGAG
GTGTTGGGAATCTGGAGATGATCCATGGGTAGAACATGCCAAGTGGTTTC
CAAGGTGTGAGTTCTTGATACGAATGAAAGGCCAAGAGTTTGTTGATGAG
ATTCAAGGTAGATATCCTCATCTTCTTGAACAGCTGTTGTCAACTTCAGA
TACCACTGGAGAAGAAAATGCTGACCCACCAATTATTCATTTTGGACCTG
GAGAAAGTTCTTCAGAAGATGCTGTCATGATGAATACACCTGTGGTTAAA
TCTGCCTTGGAAATGGGCTTTAATAGAGACCTGGTGAAACAAACAGTTCA
AAGTAAAATCCTGACAACTGGAGAGAACTATAAAACAGTTAATGATATTG
TGTCAGCACTTCTTAATGCTGAAGATGAAAAAAGAGAAGAGGAGAAGGAA
AAACAAGCTGAAGAAATGGCATCAGATGATTTGTCATTAATTCGGAAGAA
CAGAATGGCTCTCTTTCAACAATTGACATGTGTGCTTCCTATCCTGGATA
ATCTTTTAAAGGCCAATGTAATTAATAAACAGGAACATGATATTATTAAA
CAAAAAACACAGATACCTTTACAAGCGAGAGAACTGATTGATACCATTTT
GGTTAAAGGAAATGCTGCGGCCAACATCTTCAAAAACTGTCTAAAAGAAA
TTGACTCTACATTGTATAAGAACTTATTTGTGGATAAGAATATGAAGTAT
ATTCCAACAGAAGATGTTTCAGGTCTGTCACTGGAAGAACAATTGAGGAG
GTTGCAAGAAGAACGAACTTGTAAAGTGTGTATGGACAAAGAAGTTTCTG
TTGTATTTATTCCTTGTGGTCATCTGGTAGTATGCCAGGAATGTGCCCCT
TCTCTAAGAAAATGCCCTATTTGCAGGGGTATAATCAAGGGTACTGTTCG
TACATTTCTCTCTTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMHKT
ASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYS
TFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHK
QLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYS
SLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSEL
ARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSL
ETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDV
KCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPH
LLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGF
NRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMA
SDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPL
QARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVS
GLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPI
CRGIIKGTVRTFLS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32418
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 98102 Daltons