cDNA Clone UBC12 (yeast)

• Plasmid: pGEX6P-1-UBC12 yeast
• Parent Plasmid: pGEX6P1
• Expressed: GST UBC12
• Genbank: 851015
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGTTGAAATTACGCCAATTACAAAAGAAAAAGCAAAAAGAAAATGAAAACAGCAGTTCTATTCAGCCTAATTTATCGGCAGCAAGAATTCGTTTGAAAAGGGACCTTGATTCGCTGGACCTCCCCCAACAGTGACTTTGAATGTAATAACATCACCAGATAGTGCGGATAGATCACAATCGCCGAAATTGGAGGTAAT
TGTTAGGCCTGATGAAGGATATTATAATTATGGATCTATTAATTTCAATTTGGATTTTAACGAAGTTTATCCGATTGAACCACCAAAAGTTGTATGTTTGAAGAAGATCTTTCATCCAAATATCGATTTAAAGGGGAACGTTTGCCTAAATATTCTTCGAGAAGACTGGTCGCCAGCTCTAGATTTGCAGAGTATTATAACCGGGCTTCTTTTCTTGTTCTTGGAGCCTAATCCCAATGACCCGTTAAAC
AAGGATGCAGCAAAGTTACTGTGTGAGGGTGAGAAAGAATTTGCTGAAGCTGTGAGATTGACCATGTCTGGAGGTTCAATTGAACATGTCAAATATGATAACATAGTTTCTCCTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLKLRQLQKKKQKENENSS
SIQPNLSAARIRLKRDLDSLDLPPTVTLNVITSPDSADRSQSPKLEVIVR
PDEGYYNYGSINFNLDFNEVYPIEPPKVVCLKKIFHPNIDLKGNVCLNIL
REDWSPALDLQSIITGLLFLFLEPNPNDPLNKDAAKLLCEGEKEFAEAVR
LTMSGGSIEHVKYDNIVSP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32481
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 48022.89 Daltons