cDNA Clone BIRC2

• Plasmid: pGEX6P-1-BIRC2 M402A F404A
• Parent Plasmid: pGEX6P2
• Expressed: GST BIRC2 M402A F404A
• Genbank: NP_001157.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGCACAAAACTGCCTCCCAAAGACTTTTCCCAGGTCCCTC
GTATCAAAACATTAAGAGTATAATGGAAGATAGCACGATCTTGTCAGATT
GGACAAACAGCAACAAACAAAAAATGAAGTATGACTTTTCCTGTGAACTC
TACAGAATGTCTACATATTCAACTTTCCCCGCCGGGGTGCCTGTCTCAGA
AAGGAGTCTTGCTCGTGCTGGTTTTTATTATACTGGTGTGAATGACAAGG
TCAAATGCTTCTGTTGTGGCCTGATGCTGGATAACTGGAAACTAGGAGAC
AGTCCTATTCAAAAGCATAAACAGCTATATCCTAGCTGTAGCTTTATTCA
GAATCTGGTTTCAGCTAGTCTGGGATCCACCTCTAAGAATACGTCTCCAA
TGAGAAACAGTTTTGCACATTCATTATCTCCCACCTTGGAACATAGTAGC
TTGTTCAGTGGTTCTTACTCCAGCCTTTCTCCAAACCCTCTTAATTCTAG
AGCAGTTGAAGACATCTCTTCATCGAGGACTAACCCCTACAGTTATGCAA
TGAGTACTGAAGAAGCCAGATTTCTTACCTACCATATGTGGCCATTAACT
TTTTTGTCACCATCAGAATTGGCAAGAGCTGGTTTTTATTATATAGGACC
TGGAGATAGGGTAGCCTGCTTTGCCTGTGGTGGGAAGCTCAGTAACTGGG
AACCAAAGGATGATGCTATGTCAGAACACCGGAGGCATTTTCCCAACTGT
CCATTTTTGGAAAATTCTCTAGAAACTCTGAGGTTTAGCATTTCAAATCT
GAGCATGCAGACACATGCAGCTCGAATGAGAACATTTATGTACTGGCCAT
CTAGTGTTCCAGTTCAGCCTGAGCAGCTTGCAAGTGCTGGTTTTTATTAT
GTGGGTCGCAATGATGATGTCAAATGCTTTTGTTGTGATGGTGGCTTGAG
GTGTTGGGAATCTGGAGATGATCCATGGGTAGAACATGCCAAGTGGTTTC
CAAGGTGTGAGTTCTTGATACGAATGAAAGGCCAAGAGTTTGTTGATGAG
ATTCAAGGTAGATATCCTCATCTTCTTGAACAGCTGTTGTCAACTTCAGA
TACCACTGGAGAAGAAAATGCTGACCCACCAATTATTCATTTTGGACCTG
GAGAAAGTTCTTCAGAAGATGCTGTCATGATGAATACACCTGTGGTTAAA
TCTGCCTTGGAAGCGGGCGCTAATAGAGACCTGGTGAAACAAACAGTTCA
AAGTAAAATCCTGACAACTGGAGAGAACTATAAAACAGTTAATGATATTG
TGTCAGCACTTCTTAATGCTGAAGATGAAAAAAGAGAAGAGGAGAAGGAA
AAACAAGCTGAAGAAATGGCATCAGATGATTTGTCATTAATTCGGAAGAA
CAGAATGGCTCTCTTTCAACAATTGACATGTGTGCTTCCTATCCTGGATA
ATCTTTTAAAGGCCAATGTAATTAATAAACAGGAACATGATATTATTAAA
CAAAAAACACAGATACCTTTACAAGCGAGAGAACTGATTGATACCATTTT
GGTTAAAGGAAATGCTGCGGCCAACATCTTCAAAAACTGTCTAAAAGAAA
TTGACTCTACATTGTATAAGAACTTATTTGTGGATAAGAATATGAAGTAT
ATTCCAACAGAAGATGTTTCAGGTCTGTCACTGGAAGAACAATTGAGGAG
GTTGCAAGAAGAACGAACTTGTAAAGTGTGTATGGACAAAGAAGTTTCTG
TTGTATTTATTCCTTGTGGTCATCTGGTAGTATGCCAGGAATGTGCCCCT
TCTCTAAGAAAATGCCCTATTTGCAGGGGTATAATCAAGGGTACTGTTCG
TACATTTCTCTCTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMHKTASQR
LFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPA
GVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYP
SCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSP
NPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAG
FYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLR
FSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFC
CDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQ
LLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEAGANRDL
VKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDL
SLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARE
LIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSL
EEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGI
IKGTVRTFLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32695
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 97564 Daltons