cDNA Clone BMH2 (yeast)

• Plasmid: pTRC-His BMH2
• Parent Plasmid: pTRC
• Genbank: CAA59275
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

DNA sequence of insert GGATCCATGTCCCAAACTCGTGAAGATTCTGTTTACCTAGCTAAATTAGC
TGAACAAGCCGAACGTTATGAAGAAATGGTCGAAAACATGAAGGCCGTTG
CTTCATCAGGTCAAGAGTTATCTGTCGAAGAACGGAATCTATTGTCGGTT
GCTTACAAGAACGTCATCGGTGCTCGCCGTGCTTCATGGAGAATAGTTTC
TTCGATCGAACAAAAAGAAGAATCAAAGGAGAAATCTGAACATCAAGTTG
AATTAATCCGTTCTTACCGTTCTAAAATTGAAACTGAATTGACCAAAATC
TCTGACGACATTTTATCTGTGTTAGATTCTCATTTAATCCCTTCTGCTAC
TACTGGTGAGTGTAAAGTATTTTACTATAAGATGAAGGGTGACTACCACC
GTTATTTAGCTGAATTTTCCAGCGGAGATGCAAGAGAAAAGGCAACCAAC
TCCTCTTTGGAGGCTTATAAAACCGCTTCCGAAATCGCCACAACTGAATT
GCCTCCAACTCACCCAATTCGTTTATTTCTAGCTTTGAATTTCTCCGTCT
TCTATTACGAAATTCAAAACTCTCCTGATAAGGCTTGCCACTTGGCCAAA
CAAGCCTTTGATGATGCTATTGCTGAGTTAGATACTTTATCTGAAGAATC
ATACAAGGATAGCACTTTGATCATGCAATTATTAAGGGACAACTTGACCT
TATGGACCTCTGATATTTCTGAATCTGGTCAAGAAGATCAACAACAACAA
CAACAACAGCAACAGCAACAGCAACAACAGCAACAACAAGCTCCAGCTGA
ACAAACTCAAGGTGAACCAACCAAATAAGAATTC

Amino Acid Sequence

MGGSHHHHHHGMASMTGGQQMGRTLYDDDDKDRWGSMSQTREDSVYLAKL
AEQAERYEEMVENMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIV
SSIEQKEESKEKSEHQVELIRSYRSKIETELTKISDDILSVLDSHLIPSA
TTGECKVFYYKMKGDYHRYLAEFSSGDAREKATNSSLEAYKTASEIATTE
LPPTHPIRLFLALNFSVFYYEIQNSPDKACHLAKQAFDDAIAELDTLSEE
SYKDSTLIMQLLRDNLTLWTSDISESGQEDQQQQQQQQQQQQQQQQQAPA
EQTQGEPTK*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU384
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression; Calc Mol Weight 35182 Da