cDNA Clone CHK1

• Synonyms: CHEK1
• Plasmid: pGEX-CHK1
• Parent Plasmid: pGEX6P1
• Expressed: GST-CHK1
• Genbank: BC004202
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACCCT
GGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAGTAACTG
AAGAAGCAGTCGCAGTGAAGATTGTAGATATGAAGCGTGCCGTAGACTGT
CCAGAAAATATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCATGA
AAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAATATT
TATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGCCA
GACATAGGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTCAT
GGCAGGGGTGGTTTATCTGCATGGTATTGGAATAACTCACAGGGATATTA
AACCAGAAAATCTTCTGTTGGATGAAAGGGATAACCTCAAAATCTCAGAC
TTTGGCTTGGCAACAGTATTTCGGTATAATAATCGTGAGCGTTTGTTGAA
CAAGATGTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAGAGAA
GAGAATTTCATGCAGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTT
ACTGCAATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGCTG
TCAGGAGTATTCTGACTGGAAAGAAAAAAAAACATACCTCAACCCTTGGA
AAAAAATCGATTCTGCTCCTCTAGCTCTGCTGCATAAAATCTTAGTTGAG
AATCCATCAGCAAGAATTACCATTCCAGACATCAAAAAAGATAGATGGTA
CAACAAACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCAGGTG
GTGTGTCAGAGTCTCCCAGTGGATTTTCTAAGCACATTCAATCCAATTTG
GACTTCTCTCCAGTAAACAGTGCTTCTAGTGAAGAAAATGTGAAGTACTC
CAGTTCTCAGCCAGAACCCCGCACAGGTCTTTCCTTATGGGATACCAGCC
CCTCATACATTGATAAATTGGTACAAGGGATCAGCTTTTCCCAGCCCACA
TGTCCTGATCATATGCTTTTGAATAGTCAGTTACTTGGCACCCCAGGATC
CTCACAGAACCCCTGGCAGCGGTTGGTCAAAAGAATGACACGATTCTTTA
CCAAATTGGATGCAGACAAATCTTATCAATGCCTGAAAGAGACTTGTGAG
AAGTTGGGCTATCAATGGAAGAAAAGTTGTATGAATCAGGTTACTATATC
AACAACTGATAGGAGAAACAATAAACTCATTTTCAAAGTGAATTTGTTAG
AAATGGATGATAAAATATTGGTTGACTTCCGGCTTTCTAAGGGTGATGGA
TTGGAGTTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTGATTGATAT
TGTGAGCAGCCAGAAGGTTTGGCTTCCTGCCACATGAGCGGCCGC

Amino Acid Sequence

EFMAVPFVEDWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDC
PENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEP
DIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISD
FGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVL
TAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVE
NPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNL
DFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPT
CPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCE
KLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDG
LEFKRHFLKIKGKLIDIVSSQKVWLPAT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU506
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.