cDNA Clone WDR4

• Plasmid: pET28a WDR4
• Parent Plasmid: pET28a original
• Expressed: 6His-WDR4
• Genbank: BC001074
• Tag: 6His
• Species: Human

Sequence of Insert

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSATMYPYDVPDYALLDV
AVSPDDRFILTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRISVVPTQP
GLLLSSSGDGTLRLWEYRSGRQLHCCHLASLQELVDPQAPQKFAASRIAF
WCQENCVALLCDGTSVVYIFQLDARRQQLVYRQQLAFQHQVWDVAFEETQ
GLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLKKVSGVLRGNWAMLEGSA
GADASFSSLYKATFDNVTSYLKKKEERLQQQLEKKQRRRSPPPGPDGHAK
KMRPGEATLSC*

Amino Acid Sequence

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCTGTTAGA
TGTGGCTGTGAGTCCTGATGACCGCTTCATCCTCACTGCCGACCGGGACG
AGAAGATCCGAGTCAGCTGGGCCGCGGCGCCCCATAGCATCGAGTCCTTC
TGCTTGGGGCACACAGAGTTTGTGAGCCGTATCTCCGTGGTGCCAACTCA
GCCCGGGCTGCTTCTGTCCTCCTCTGGGGACGGCACCCTGAGGCTCTGGG
AGTACAGGAGCGGCCGCCAGCTGCACTGCTGTCACCTGGCCAGTCTGCAG
GAGCTGGTGGACCCCCAGGCCCCCCAGAAGTTTGCCGCGTCCAGGATTGC
ATTCTGGTGCCAGGAGAACTGCGTGGCGCTCCTGTGCGACGGCACTTCTG
TGGTCTACATCTTCCAGCTGGACGCCCGCAGACAGCAGTTGGTGTACAGG
CAGCAGCTGGCGTTCCAGCACCAAGTGTGGGACGTGGCTTTCGAGGAGAC
CCAGGGGCTGTGGGTGCTCCAGGACTGCCAGGAAGCCCCCCTGGTGCTCT
ACAGGCCTGTGGGCGACCAGTGGCAGTCTGTTCCTGAGAGCACCGTGTTA
AAGAAAGTCTCTGGTGTTCTTCGTGGGAACTGGGCCATGCTGGAAGGCTC
TGCCGGCGCAGACGCCAGCTTCAGCAGTCTCTACAAGGCCACGTTCGACA
ACGTGACCTCCTACCTGAAGAAGAAAGAGGAGAGACTGCAGCAGCAGCTA
GAGAAGAAGCAGCGGCGCCGGAGTCCCCCGCCTGGGCCCGACGGGCATGC
CAAGAAGATGAGACCGGGGGAGGCGACGCTAAGTTGCTGAGGATCC

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU812
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct with N terminal 6His and T7 tags