cDNA Clone CSK

• Plasmid: pGEX4T1 CSK
• Parent Plasmid: pGEX4T1
• Expressed: GST Thrombin CSK
• Genbank: NM_004383
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCAGCAATACAGGCCGCCTGGCCATCCGGTACAGAATGTAT
TGCCAAGTACAACTTCCACGGCACTGCCGAGCAGGACCTGCCCTTCTGCA
AAGGAGACGTGCTCACCATTGTGGCCGTCACCAAGGACCCCAACTGGTAC
AAAGCCAAAAACAAGGTGGGCCGTGAGGGCATCATCCCAGCCAACTACGT
CCAGAAGCGGGAGGGCGTGAAGGCGGGTACCAAACTCAGCCTCATGCCTT
GGTTCCACGGCAAGATCACACGGGAGCAGGCTGAGCGGCTTCTGTACCCG
CCGGAGACAGGCCTGTTCCTGGTGCGGGAGAGCACCAACTACCCCGGAGA
CTACACGCTGTGCGTGAGCTGCGACGGCAAGGTGGAGCACTACCGCATCA
TGTACCATGCCAGCAAGCTCAGCATCGACGAGGAGGTGTACTTTGAGAAC
CTCATGCAGCTGGTGGAGCACTACACCTCAGACGCAGATGGACTCTGTAC
GCGCCTCATTAAACCAAAGGTCATGGAGGGCACAGTGGCGGCCCAGGATG
AGTTCTACCGCAGCGGCTGGGCCCTGAACATGAAGGAGCTGAAGCTGCTG
CAGACCATCGGGAAGGGGGAGTTCGGAGACGTGATGCTGGGCGATTACCG
AGGGAACAAAGTCGCCGTCAAGTGCATTAAGAACGACGCCACTGCCCAGG
CCTTCCTGGCTGAAGCCTCAGTCATGACGCAACTGCGGCATAGCAACCTG
GTGCAGCTCCTGGGCGTGATCGTGGAGGAGAAGGGCGGGCTCTACATCGT
CACTGAGTACATGGCCAAGGGGAGCCTTGTGGACTACCTGCGGTCTAGGG
GTCGGTCAGTGCTGGGCGGAGACTGTCTCCTCAAGTTCTCGCTAGATGTC
TGCGAGGCCATGGAATACCTGGAGGGCAACAATTTCGTGCATCGAGACCT
GGCTGCCCGCAATGTGCTGGTGTCTGAGGACAACGTGGCCAAGGTCAGCG
ACTTTGGTCTCACCAAGGAGGCGTCCAGCACCCAGGACACGGGCAAGCTG
CCAGTCAAGTGGACAGCCCCTGAGGCCCTGAGAGAGAAGAAATTCTCCAC
TAAGTCTGACGTGTGGAGTTTCGGAATCCTTCTCTGGGAAATCTACTCCT
TTGGGCGAGTGCCTTATCCAAGAATTCCCCTGAAGGACGTCGTCCCTCGG
GTGGAGAAGGGCTACAAGATGGATGCCCCCGACGGCTGCCCGCCCGCAGT
CTATGAAGTCATGAAGAACTGCTGGCACCTGGACGCCGCCATGCGGCCCT
CCTTCCTACAGCTCCGAGAGCAGCTTGAGCACATCAAAACCCACGAGCTG
CACCTGTGACGGCTGGCCTCCGCCTGGGTCATGGGCCTGTGGGGACTGAA
CCTGGAAGATCATGGACGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMSAIQAAWPSGTECIAKYNFHGTA
EQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAG
TKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDG
KVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVME
GTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCI
KNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSL
VDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSE
DNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGI
LLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWH
LDAAMRPSFLQLREQLEHIKTHELHL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1500
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterially expressed N terminal GST fusion
• Low Temp: No