cDNA Clone VASP

• Plasmid: pEGFP-C1-VASP
• Parent Plasmid: pEGFPC1
• Expressed: GFP-VASP
• Genbank: CAA67147
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCAGCGAG
ACGGTCATCTGTTCCAGCCGGGCCACTGTGATGCTTTATGATGATGGCAA
CAAGCGATGGCTCCCTGCTGGCACGGGTCCCCAGGCCTTCAGCCGCGTCC
AGATCTACCACAACCCCACGGCCAATTCCTTTCGCGTCGTGGGCCGGAAG
ATGCAGCCCGACCAGCAGGTGGTCATCAACTGTGCCATCGTCCGGGGTGT
CAAGTATAACCAGGCCACCCCCAACTTCCATCAGTGGCGCGACGCTCGCC
AGGTCTGGGGCCTCAACTTCGGCAGCAAGGAGGATGCGGCCCAGTTTGCC
GCCGGCATGGCCAGTGCCCTAGAGGCGTTGGAAGGAGGTGGGCCCCCTCC
ACCCCCAGCACTTCCCACCTGGTCGGTCCCGAACGGCCCCTCCCCGGAGG
AGGTGGAGCAGCAGAAAAGGCAGCAGCCCGGCCCGTCGGAGCACATAGAG
CGCCGGGTCTCCAATGCAGGAGGCCCACCTGCTCCCCCCGCTGGGGGTCC
ACCCCCACCACCAGGACCTCCCCCTCCTCCAGGTCCCCCCCCACCCCCAG
GTTTGCCCCCTTCGGGGGTCCCAGCTGCAGCGCACGGAGCAGGGGGAGGA
CCACCCCCTGCACCCCCTCTCCCGGCAGCACAGGGCCCTGGTGGTGGGGG
AGCTGGGGCCCCAGGCCTGGCCGCAGCTATTGCTGGAGCCAAACTCAGGA
AAGTCAGCAAGCAGGAGGAGGCCTCAGGGGGGCCCACAGCCCCCAAAGCT
GAGAGTGGTCGAAGCGGAGGTGGGGGACTCATGGAAGAGATGAACGCCAT
GCTGGCCCGGAGAAGGAAAGCCACGCAAGTTGGGGAGAAAACCCCCAAGG
ATGAATCTGCCAATCAGGAGGAGCCAGAGGCCAGAGTCCCGGCCCAGAGT
GAATCTGTGCGGAGACCCTGGGAGAAGAACAGCACAACCTTGCCAAGGAT
GAAGTCGTCTTCTTCGGTGACCACTTCCGAGACCCAACCCTGCACGCCCA
GCTCCAGTGATTACTCGGACCTACAGAGGGTGAAACAGGAGCTTCTGGAA
GAGGTGAAGAAGGAATTGCAGAAAGTGAAAGAGGAAATCATTGAAGCCTT
CGTCCAGGAGCTGAGGAAGCGGGGTTCTCCCTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTGSATMYPYDVPDYASETVICSSRATVMLYDDGNKRWLP
AGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQA
TPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALP
TWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPG
PPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPG
LAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRR
KATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSS
VTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELR
KRGSP*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU1556
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tag for mammalian expression