cDNA Clone CHK1

• Synonyms: CHEK1
• Plasmid: pFBHTa CHK1 D290-476
• Parent Plasmid: pSC-A
• Expressed: 6His-CHK1 D290-476
• Genbank: BC004202
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACCCT
GGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAGTAACTG
AAGAAGCAGTCGCAGTGAAGATTGTAGATATGAAGCGTGCCGTAGACTGT
CCAGAAAATATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCATGA
AAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAATATT
TATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGCCA
GACATAGGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTCAT
GGCAGGGGTGGTTTATCTGCATGGTATTGGAATAACTCACAGGGATATTA
AACCAGAAAATCTTCTGTTGGATGAAAGGGATAACCTCAAAATCTCAGAC
TTTGGCTTGGCAACAGTATTTCGGTATAATAATCGTGAGCGTTTGTTGAA
CAAGATGTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAGAGAA
GAGAATTTCATGCAGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTT
ACTGCAATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGCTG
TCAGGAGTATTCTGACTGGAAAGAAAAAAAAACATACCTCAACCCTTGGA
AAAAAATCGATTCTGCTCCTCTAGCTCTGCTGCATAAAATCTTAGTTGAG
AATCCATCAGCAAGAATTACCATTCCAGACATCAAAAAAGATAGATGGTA
CAACAAACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCAGGTG
GTGTGTCAGAGTCTCCCAGTGGATGAGCGGCCGCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMAVPFVEDWDLVQTLGEGAY
GEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKF
YGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVY
LHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGT
LPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSD
WKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLK
KGAKRPRVTSGGVSESPSG*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1599
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for Baculovirus expression of N terminal 6His tag