cDNA Clone CBS

• Plasmid: pGEX6P-1-HA CBS
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA CBS
• Genbank: P35520
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCCTTCTGA
GACCCCCCAGGCAGAAGTGGGGCCCACAGGCTGCCCCCACCGCTCAGGGC
CACACTCGGCGAAGGGGAGCCTGGAGAAGGGGTCCCCAGAGGATAAGGAA
GCCAAGGAGCCCCTGTGGATCCGGCCCGATGCTCCGAGCAGGTGCACCTG
GCAGCTGGGCCGGCCTGCCTCCGAGTCCCCACATCACCACACTGCCCCGG
CAAAATCTCCAAAAATCTTGCCAGATATTCTGAAGAAAATCGGGGACACC
CCTATGGTCAGAATCAACAAGATTGGGAAGAAGTTCGGCCTGAAGTGTGA
GCTCTTGGCCAAGTGTGAGTTCTTCAACGCGGGCGGGAGCGTGAAGGACC
GCATCAGCCTGCGGATGATTGAGGATGCTGAGCGCGACGGGACGCTGAAG
CCCGGGGACACGATTATCGAGCCGACATCCGGGAACACCGGGATCGGGCT
GGCCCTGGCTGCGGCAGTGAGGGGCTATCGCTGCATCATCGTGATGCCAG
AGAAGATGAGCTCCGAGAAGGTGGACGTGCTGCGGGCACTGGGGGCTGAG
ATTGTGAGGACGCCCACCAATGCCAGGTTCGACTCCCCGGAGTCACACGT
GGGGGTGGCCTGGCGGCTGAAGAACGAAATCCCCAATTCTCACATCCTAG
ACCAGTACCGCAACGCCAGCAACCCCCTGGCTCACTATGACACCACCGCT
GATGAGATCCTGCAGCAGTGTGATGGGAAGCTGGACATGCTGGTGGCTTC
AGTGGGCACGGGCGGCACCATCACGGGCATTGCCAGGAAGCTGAAGGAGA
AGTGTCCTGGATGCAGGATCATTGGGGTGGATCCCGAAGGGTCCATCCTC
GCAGAGCCGGAGGAGCTGAACCAGACGGAGCAGACAACCTACGAGGTGGA
AGGGATCGGCTACGACTTCATCCCCACGGTGCTGGACAGGACGGTGGTGG
ACAAGTGGTTCAAGAGCAACGATGAGGAGGCGTTCACCTTTGCCCGCATG
CTGATCGCGCAAGAGGGGCTGCTGTGCGGTGGCAGTGCTGGCAGCACGGT
GGCGGTGGCCGTGAAGGCCGCGCAGGAGCTGCAGGAGGGCCAGCGCTGCG
TGGTCATTCTGCCCGACTCAGTGCGGAACTACATGACCAAGTTCCTGAGC
GACAGGTGGATGCTGCAGAAGGGCTTTCTGAAGGAGGAGGACCTCACGGA
GAAGAAGCCCTGGTGGTGGCACCTCCGTGTTCAGGAGCTGGGCCTGTCAG
CCCCGCTGACCGTGCTCCCGACCATCACCTGTGGGCACACCATCGAGATC
CTCCGGGAGAAGGGCTTCGACCAGGCGCCCGTGGTGGATGAGGCGGGGGT
AATCCTGGGAATGGTGACGCTTGGGAACATGCTCTCGTCCCTGCTTGCCG
GGAAGGTGCAGCCGTCAGACCAAGTTGGCAAAGTCATCTACAAGCAGTTC
AAACAGATCCGCCTCACGGACACGCTGGGCAGGCTCTCGCACATCCTGGA
GATGGACCACTTCGCCCTGGTGGTGCACGAGCAGATCCAGTACCACAGCA
CCGGGAAGTCCAGTCAGCGGCAGATGGTGTTCGGGGTGGTCACCGCCATT
GACTTGCTGAACTTCGTGGCCGCCCAGGAGCGGGACCAGAAGTGAGAATT
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFATMYPYDVPDYAPSET
PQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPSRCTWQ
LGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFGLKCEL
LAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPTSGNTGIGLA
LAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVG
VAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASV
GTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEG
IGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVA
VAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEK
KPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVI
LGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEM
DHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1815
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA tag for bacterial expression.