cDNA Clone VPS15

• Plasmid: pMal VPS15 433-667
• Parent Plasmid: pMALP2X
• Expressed: MBP-VPS15 433-667
• Genbank: NP_055417
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCTCT
CGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACATTC
TGCCAGGCATAGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTA
GCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACAGCTCTGAGATTCCT
GGAATTAGTACAGTTAAAAAATCTCAATATGGAAAATGACCCCAATAATG
AAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTC
CAAGCCTTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGTGA
CCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGAATAACACGGC
TGTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCAC
ATGATTACTTTCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATT
TTTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGCTCCT
CAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTT
GTCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACT
GCTACAAAAACCCCATGTTTACGAATTTGCCAGTGATATTGCCCCCTTCC
TGTGTCATCCCTAAGGATCC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFGSVRAEALRTLTKVLALVKEVPRNDINIYPEYIL
PGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLNMENDPNNE
EIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRL
CVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSS
ILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEFASDIAPFL
CHP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1928
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal MBP tag for bacterial expression.; Estimated MW of expressed protein : 72088 Daltons