cDNA Clone Ubiquitin

• Plasmid: pGEX6P1 Ubiquitin
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C Ubiquitin
• Genbank: P62988
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 Ubiquitin 52.96 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1939
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression. Insert was cloned in BamH1-Xho1 into BamH1-Sal1. However, insert can be excised BamH1-Xho1 or BamH1-Not1. See attached SnapGene file for full details.
• Low Temp: No