cDNA Clone CRTC2

• Synonyms: TORC2
• Plasmid: pGEX6P-2-CRTC2
• Parent Plasmid: pGEX6P2
• Expressed: GST-CRTC2
• Genbank: NM_181715
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGACGTCGGGGGCGAACGGGCCTGGTTCGGCCACGGC
CTCGGCTTCCAATCCGCGCAAATTTAGTGAGAAGATTGCGCTGCAGAAGC
AGCGTCAGGCCGAGGAGACGGCGGCCTTCGAGGAGGTGATGATGGACATC
GGCTCCACCCGGTTACAGGCCCAAAAACTGCGACTGGCATACACAAGGAG
CTCTCATTATGGTGGGTCTCTGCCCAATGTTAACCAGATTGGCTCTGGCC
TGGCCGAGTTCCAGAGCCCCCTCCACTCACCTTTGGATTCATCTCGGAGC
ACTCGGCACCATGGGCTGGTGGAACGGGTGCAGCGAGATCCTCGAAGAAT
GGTGTCCCCACTTCGCCGATACACCCGCCACATTGACAGCTCTCCCTATA
GTCCTGCCTACTTATCTCCTCCCCCAGAGTCTAGCTGGCGAAGGACGATG
GCCTGGGGCAATTTCCCTGCAGAGAAGGGGCAGTTGTTTCGACTACCATC
TGCACTTAACAGGACAAGCTCTGACTCTGCCCTTCATACAAGTGTGATGA
ACCCCAGTCCCCAGGATACCTACCCAGGCCCCACACCTCCCAGCATCCTG
CCCAGCCGACGTGGGGGTATTCTGGATGGTGAAATGGACCCCAAAGTACC
TGCTATTGAGGAGAACTTGCTAGATGACAAGCATTTGCTGAAGCCATGGG
ATGCTAAGAAGCTATCCTCATCCTCTTCCCGACCTCGGTCCTGTGAAGTC
CCTGGAATTAACATCTTTCCATCTCCTGACCAGCCTGCCAATGTGCCTGT
CCTCCCACCTGCCATGAACACGGGGGGCTCCCTACCTGACCTCACCAACC
TGCACTTTCCCCCACCACTGCCCACCCCCCTGGACCCTGAAGAGACAGCC
TACCCTAGCCTGAGTGGGGGCAACAGTACCTCCAATTTGACCCACACCAT
GACTCACCTGGGCATCAGCAGGGGCATGGGCCTGGGCCCAGGCTATGATG
CACCAGGACTTCATTCACCTCTCAGCCACCCATCCCTGCAGTCCTCCCTA
AGCAATCCCAACCTCCAGGCTTCCCTGAGCAGTCCTCAGCCCCAGCTTCA
GGGCTCCCACAGCCACCCCTCTCTGCCTGCCTCCTCCTTGGCCCGCCATG
TACTGCCCACCACCTCCCTGGGCCACCCCTCACTCAGTGCTCCGGCTCTT
TCCTCCTCCTCTTCCTCCTCCTCCACTTCATCTCCTGTTTTGGGCGCCCC
CTCTTACCCTGCTTCTACCCCTGGGGCCTCCCCCCACCACCGCCGTGTGC
CCCTCAGCCCCCTGAGTTTGCTCGCGGGCCCAGCCGACGCCAGAAGGTCC
CAACAGCAGCTGCCCAAACAGTTTTCGCCAACAATGTCACCCACCTTGTC
TTCCATCACTCAGGGCGTCCCCCTGGATACCAGTAAACTGTCCACTGACC
AGCGGTTACCCCCATACCCATACAGCTCCCCAAGTCTGGTTCTGCCTACC
CAGCCCCACACCCCAAAGTCTCTACAGCAGCCAGGGCTGCCCTCTCAGTC
TTGTTCAGTGCAGTCCTCAGGTGGGCAGCCCCCAGGCAGGCAGTCTCATT
ATGGGACACCGTACCCACCTGGGCCCAGTGGGCATGGGCAACAGTCTTAC
CACCGGCCAATGAGTGACTTCAACCTGGGGAATCTGGAGCAGTTCAGCAT
GGAGAGCCCATCAGCCAGCCTGGTGCTGGATCCCCCTGGCTTTTCTGAAG
GGCCTGGATTTTTAGGGGGTGAGGGGCCAATGGGTGGCCCCCAGGATCCC
CACACCTTCAACCACCAGAACTTGACCCACTGTTCCCGCCATGGCTCAGG
GCCTAACATCATCCTCACAGGGGACTCCTCTCCAGGTTTCTCTAAGGAGA
TTGCAGCAGCCCTGGCCGGAGTGCCTGGCTTTGAGGTGTCAGCAGCTGGA
TTGGAGCTAGGGCTTGGGCTAGAAGATGAGCTGCGCATGGAGCCACTGGG
CCTGGAAGGGCTAAACATGCTGAGTGACCCCTGTGCCCTGCTGCCTGATC
CTGCTGTGGAGGAGTCATTCCGCAGTGACCGGCTCCAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMATSGANG
PGSATASASNPRKFSEKIALQKQRQAEETAAFEEVMMDIGSTRLQAQKLR
LAYTRSSHYGGSLPNVNQIGSGLAEFQSPLHSPLDSSRSTRHHGLVERVQ
RDPRRMVSPLRRYTRHIDSSPYSPAYLSPPPESSWRRTMAWGNFPAEKGQ
LFRLPSALNRTSSDSALHTSVMNPSPQDTYPGPTPPSILPSRRGGILDGE
MDPKVPAIEENLLDDKHLLKPWDAKKLSSSSSRPRSCEVPGINIFPSPDQ
PANVPVLPPAMNTGGSLPDLTNLHFPPPLPTPLDPEETAYPSLSGGNSTS
NLTHTMTHLGISRGMGLGPGYDAPGLHSPLSHPSLQSSLSNPNLQASLSS
PQPQLQGSHSHPSLPASSLARHVLPTTSLGHPSLSAPALSSSSSSSSTSS
PVLGAPSYPASTPGASPHHRRVPLSPLSLLAGPADARRSQQQLPKQFSPT
MSPTLSSITQGVPLDTSKLSTDQRLPPYPYSSPSLVLPTQPHTPKSLQQP
GLPSQSCSVQSSGGQPPGRQSHYGTPYPPGPSGHGQQSYHRPMSDFNLGN
LEQFSMESPSASLVLDPPGFSEGPGFLGGEGPMGGPQDPHTFNHQNLTHC
SRHGSGPNIILTGDSSPGFSKEIAAALAGVPGFEVSAAGLELGLGLEDEL
RMEPLGLEGLNMLSDPCALLPDPAVEESFRSDRLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3476
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression