cDNA Clone BirA

• Plasmid: pcDNA3.1+ 3HA BirA
• Parent Plasmid: pcDNA3.1+(neo)
• Expressed: 3HA BirA
• Genbank: ECOBIRA
• Tag: HA
• Species: Escherichia coli

Sequence of Insert

GAATTCGCCGCCACCATGGCCACCTATGACGTCCCAGATTACGCAAGTTT
GCCCGGGTATGATGTTCCTGATTATGCTAGCCTCCCGGGTTACGATGTGC
CCGACTATGCCTCACTTCCAGGCAGATCTATGAAGGATAACACCGTGCCA
CTGAAATTGATTGCCCTGTTAGCGAACGGTGAATTTCACTCTGGCGAGCA
GTTGGGTGAAACGCTGGGAATGAGCCGGGCGGCTATTAATAAACACATTC
AGACACTGCGTGACTGGGGCGTTGATGTCTTTACCGTTCCGGGTAAAGGA
TACAGCCTGCCTGAGCCTATCCAGTTACTTAATGCTAAACAGATATTGGG
TCAGCTGGATGGCGGTAGTGTAGCCGTGCTGCCAGTGATTGACTCCACGA
ATCAGTACCTTCTTGATCGTATCGGAGAGCTTAAATCGGGCGATGCTTGC
ATTGCAGAATACCAGCAGGCTGGCCGTGGTCGCCGGGGTCGGAAATGGTT
TTCGCCTTTTGGCGCAAACTTATATTTGTCGATGTTCTGGCGTCTGGAAC
AAGGCCCGGCGGCGGCGATTGGTTTAAGTCTGGTTATCGGTATCGTGATG
GCGGAAGTATTACGCAAGCTGGGTGCAGATAAAGTTCGTGTTAAATGGCC
TAATGACCTCTATCTGCAGGATCGCAAGCTGGCAGGCATTCTGGTGGAGC
TGACTGGCAAAACTGGCGATGCGGCGCAAATAGTCATTGGAGCCGGGATC
AACATGGCAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGAT
CACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCA
TGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGA
TTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCG
CCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCG
GAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAA
CCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAACTAG
T

Amino Acid Sequence

MATYDVPDYASLPGYDVPDYASLPGYDVPDYASLPGRSMKDNTVPLKLIA
LLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPE
PIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQ
QAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLR
KLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMR
RVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYL
SRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGG
EISLRSAEK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3668
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 3HA tag for mammalian expression.