cDNA Clone VPS34

• Plasmid: pFBHTa HA VPS34 D761A
• Parent Plasmid: pSC-A
• Expressed: 6His-HA VPS34 D761R
• Genbank: NP_002638
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGGGAAGC
AGAGAAGTTTCACTACATCTATAGTTGTGACCTGGATATCAACGTCCAGC
TTAAGATAGGAAGCTTGGGAGGGAAGAGAGAACAAAAGAGTTATAAAGCT
GTCCTGGAAGACCCAATGTTGAAGTTCTCAGGACTATATCAAGAGACATG
CTCTGATCTTTATGTTACTTGTCAAGTTTTTGCAGAAGGGAAGCCTTTGG
CCTTGCCAGTGAGAACATCCTACAAAGCATTTAGTACAAGATGGAACTGG
AATGAATGGCTGAAACTACCAGTAAAATACCCTGACCTGCCCAGGAATGC
CCAAGTGGCCCTCACCATATGGGATGTGTATGGTCCCGGAAAAGCAGTGC
CTGTAGGAGGAACAACGGTTTCGCTCTTTGGAAAATACGGCATGTTTCGC
CAAGGGATGCATGACTTGAAAGTCTGGCCTAATGTAGAAGCAGATGGATC
AGAACCCACAAAAACTCCTGGCAGAACAAGTAGCACTCTCTCAGAAGATC
AGATGAGCCGTCTTGCCAAGCTCACCAAAGCTCATCGACAAGGACACATG
GTGAAAGTAGATTGGCTGGATAGATTGACATTTAGAGAAATAGAAATGAT
AAATGAGAGTGAAAAACGAAGTTCTAATTTCATGTACCTGATGGTTGAAT
TTCGATGTGTCAAGTGTGATGATAAGGAATATGGTATTGTTTATTATGAA
AAGGACGGTGATGAATCATCTCCAATTTTAACAAGTTTTGAATTAGTGAA
AGTTCCTGACCCCCAGATGTCTATGGAGAATTTAGTTGAGAGCAAACACC
ACAAGCTTGCCCGGAGTTTAAGAAGTGGACCTTCTGACCACGATCTGAAA
CCCAATGCTGCCACGAGAGATCAGTTAAATATTATTGTGAGTTATCCACC
AACCAAGCAACTTACATATGAAGAACAAGATCTTGTTTGGAAGTTTAGAT
ATTATCTTACGAATCAAGAAAAAGCCTTGACAAAATTCTTGAAATGTGTT
AATTGGGATCTACCTCAAGAGGCCAAACAGGCCTTGGAACTTCTGGGAAA
ATGGAAGCCGATGGATGTAGAGGACTCCTTGGAGCTGTTATCCTCTCATT
ACACCAACCCAACTGTGAGGCGTTATGCTGTTGCCCGGTTGCGACAGGCC
GATGATGAGGATTTGTTGATGTACCTATTACAATTGGTCCAGGCTCTCAA
ATATGAAAATTTTGATGATATAAAGAATGGATTGGAACCTACCAAGAAGG
ATAGTCAGAGTTCAGTGTCAGAAAATGTGTCAAATTCTGGAATAAATTCT
GCAGAAATAGATAGCTCCCAAATTATAACCAGCCCCCTTCCTTCAGTCTC
TTCACCTCCTCCTGCATCAAAAACAAAAGAAGTTCCAGATGGCGAAAATC
TGGAACAAGATCTCTGTACCTTCTTGATATCGAGAGCCTGCAAAAACTCA
ACACTGGCTAATTATTTATACTGGTATGTGATAGTGGAATGTGAAGATCA
AGATACTCAGCAGAGAGATCCAAAGACCCATGAGATGTACTTGAACGTAA
TGAGAAGATTCAGCCAAGCATTGTTGAAGGGTGATAAGTCTGTCAGAGTT
ATGCGTTCTTTGCTGGCTGCACAACAGACATTTGTAGATCGGTTGGTGCA
TCTAATGAAGGCAGTACAACGCGAAAGTGGAAATCGTAAGAAAAAGAATG
AGAGACTACAGGCATTGCTTGGAGATAATGAAAAGATGAATTTGTCAGAT
GTGGAACTTATCCCGTTGCCTTTAGAACCCCAAGTGAAAATTAGAGGAAT
AATTCCGGAAACAGCTACACTGTTTAAAAGTGCCCTTATGCCTGCACAGT
TGTTTTTTAAGACGGAAGATGGAGGCAAATATCCAGTTATATTTAAGCAT
GGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCAT
GGACAAGCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATA
AGGTGTTAGCCACCAGTACAAAACATGGCTTCATGCAGTTTATCCAGTCA
GTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATTCAGAACTTTTT
TAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGG
TCATGGACACTTACGTTAAAAGCTGTGCTGGATATTGCGTGATCACCTAT
ATACTTGGAGTTGGAGACAGGCACCTGGATAACCTTTTGCTAACAAAAAC
AGGCAAACTCTTCCACATAAGATTTGGATATATTTTGGGTCGGGATCCAA
AGCCTCTTCCTCCACCAATGAAGCTGAATAAAGAAATGGTAGAAGGAATG
GGGGGCACACAGAGTGAGCAGTACCAAGAGTTCCGTAAACAGTGTTACAC
GGCTTTCCTCCACCTGCGAAGGTATTCTAATCTGATTTTGAACTTGTTTT
CCTTGATGGTTGATGCAAACATTCCAGATATTGCACTTGAACCAGATAAA
ACTGTGAAAAAGGTTCAGGATAAATTCCGCTTAGACCTGTCGGATGAAGA
GGCTGTGCATTACATGCAGAGTCTGATTGATGAGAGTGTCCATGCTCTTT
TTGCTGCAGTGGTGGAACAGATTCACAAGTTTGCCCAGTACTGGAGAAAA
TGAGAATTC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFATMYPYDVPDYAGEAEKFHY
IYSCDLDINVQLKIGSLGGKREQKSYKAVLEDPMLKFSGLYQETCSDLYV
TCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALT
IWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKT
PGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEK
RSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQ
MSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLT
YEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALELLGKWKPMD
VEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFD
DIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPA
SKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQR
DPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAV
QRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETA
TLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLR
KENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAP
SENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFH
IRFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHL
RRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYM
QSLIDESVHALFAAVVEQIHKFAQYWRK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3760
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His/HA tag for baculovirus expression.