cDNA Clone VPS34

• Plasmid: pCMV5-HA VPS34 mTOR
• Parent Plasmid: pCMV5
• Expressed: HA-VPS34 mTOR
• Genbank: NP_002638
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGGGAAGC
AGAGAAGTTTCACTACATCTATAGTTGTGACCTGGATATCAACGTCCAGC
TTAAGATAGGAAGCTTGGGAGGGAAGAGAGAACAAAAGAGTTATAAAGCT
GTCCTGGAAGACCCAATGTTGAAGTTCTCAGGACTATATCAAGAGACATG
CTCTGATCTTTATGTTACTTGTCAAGTTTTTGCAGAAGGGAAGCCTTTGG
CCTTGCCAGTGAGAACATCCTACAAAGCATTTAGTACAAGATGGAACTGG
AATGAATGGCTGAAACTACCAGTAAAATACCCTGACCTGCCCAGGAATGC
CCAAGTGGCCCTCACCATATGGGATGTGTATGGTCCCGGAAAAGCAGTGC
CTGTAGGAGGAACAACGGTTTCGCTCTTTGGAAAATACGGCATGTTTCGC
CAAGGGATGCATGACTTGAAAGTCTGGCCTAATGTAGAAGCAGATGGATC
AGAACCCACAAAAACTCCTGGCAGAACAAGTAGCACTCTCTCAGAAGATC
AGATGAGCCGTCTTGCCAAGCTCACCAAAGCTCATCGACAAGGACACATG
GTGAAAGTAGATTGGCTGGATAGATTGACATTTAGAGAAATAGAAATGAT
AAATGAGAGTGAAAAACGAAGTTCTAATTTCATGTACCTGATGGTTGAAT
TTCGATGTGTCAAGTGTGATGATAAGGAATATGGTATTGTTTATTATGAA
AAGGACGGTGATGAATCATCTCCAATTTTAACAAGTTTTGAATTAGTGAA
AGTTCCTGACCCCCAGATGTCTATGGAGAATTTAGTTGAGAGCAAACACC
ACAAGCTTGCCCGGAGTTTAAGAAGTGGACCTTCTGACCACGATCTGAAA
CCCAATGCTGCCACGAGAGATCAGTTAAATATTATTGTGAGTTATCCACC
AACCAAGCAACTTACATATGAAGAACAAGATCTTGTTTGGAAGTTTAGAT
ATTATCTTACGAATCAAGAAAAAGCCTTGACAAAATTCTTGAGATGTGTT
AATTGGGATCTACCTCAAGAGGCCAAACAGGCCTTGGAACTTCTGGGAAA
ATGGAAGCCGATGGATGTAGAGGACTCCTTGGAGCTGTTATCCTCTCATT
ACACCAACCCAACTGTGAGGCGCTATGCTGTTGCCCGGTTGCGACAGGCC
GATGATGAGGATTTGTTGATGTACCTATTACAATTGGTCCAGGCTCTCAA
ATATGAAAATTTTGATGATATAAAGAATGGATTGGAACCTACCAAGAAGG
ATAGTCAGAGTTCAGTGTCAGAAAATGTGTCAAATTCTGGAATAAATTCT
GCAGAAATAGATAGCTCCCAAATTATAACCAGCCCCCTTCCTTCAGTCTC
TTCACCTCCTCCTGCATCAAAAACAAAAGAAGTTCCAGATGGCGAAAATC
TGGAACAAGATCTCTGTACCTTCTTGATATCGAGAGCCTGCAAAAACTCA
ACACTGGCTAATTATTTATACTGGTATGTGATAGTGGAATGTGAAGATCA
AGATACTCAGCAGAGAGATCCAAAGACCCATGAGATGTACTTGAACGTAA
TGAGAAGATTCAGCCAAGCATTGTTGAAGGGTGATAAGTCTGTCAGAGTT
ATGCGTTCTTTGCTGGCTGCACAACAGACATTTGTAGATCGGTTGGTGCA
TCTAATGAAGGCAGTACAACGCGAAAGTGGAAATCGTAAGAAAAAGAATG
AGAGACTACAGGCATTGCTTGGAGATAATGAAAAGATGAATTTGTCAGAT
GTGGAACTTATCCCGTTGCCTTTAGAACCCCAAGTGAAAATTAGAGGAAT
AATTCCGGAAACAGCTACACTGTTTAAAAGTGCCCTTATGCCTGCACAGT
TGTTTTTTAAGACGGAAGATGGAGGCAAATATCCAGTTATATTTAAGCAT
GGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCAT
GGACAAGCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATA
AGGTGTTAGCCACCAGTACAAAACATGGCTTCATGCAGTTTATCCAGTCA
GTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATTCAGAACTTTTT
TAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGG
TCATGGACACTTACGTTAAAAGCTGTGCTGGATATTGCGTGATCACCTAT
ATACTTGGAGTTGGAGACAGGCACCTGGATAACCTTTTGCTAACAAAAAC
AGGCAAACTCTTCCACATAGACTTTGGATATATTTTGGGTCGGGCTATGA
CCCGAGAGAAGTTTCCAGAGAAGATTCCACCAATGAAGCTGAATAAAGAA
ATGGTAGAAGGAATGGGGGGCACACAGAGTGAGCAGTACCAAGAGTTCCG
TAAACAGTGTTACACGGCTTTCCTCCACCTGCGAAGGTATTCTAATCTGA
TTTTGAACTTGTTTTCCTTGATGGTTGATGCAAACATTCCAGATATTGCA
CTTGAACCAGATAAAACTGTGAAAAAGGTTCAGGATAAATTCCGCTTAGA
CCTGTCGGATGAAGAGGCTGTGCATTACATGCAGAGTCTGATTGATGAGA
GTGTCCATGCTCTTTTTGCTGCAGTGGTGGAACAGATTCACAAGTTTGCC
CAGTACTGGAGAAAATGAGAATTC

Amino Acid Sequence

MYPYDVPDYAGEAEKFHYIYSCDLDINVQLKIGSLGGKREQKSYKAVLED
PMLKFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWL
KLPVKYPDLPRNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMH
DLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVD
WLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGD
ESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAA
TRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLRCVNWDL
PQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDED
LLMYLLQLVQALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEID
SSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLAN
YLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSL
LAAQQTFVDRLVHLMKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELI
PLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDL
RQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVA
EVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGV
GDRHLDNLLLTKTGKLFHIDFGYILGRAMTREKFPEKIPPMKLNKEMVEG
MGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPD
KTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWR
K*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3821
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression.