cDNA Clone UBA3; APPBP1

• Synonyms: UBA3, Ubiquitin-Activating Enzyme E1 Homolog
• Plasmid: pOPC UBA3 6His, GST APPBP1
• Parent Plasmid: pOPC
• Expressed: UBA3 6His, GST APPBP1
• Genbank: NP_003959.3, NP_003896
• Tag: GST
• Species: Human

Sequence of Insert

DNA sequence of UBA3 insert
TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGGA
TGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGG
AACCATGTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCTGA
TTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGTAAAG
TTCTAGTCATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTG
GCCTTGTCTGGTTTTAGACAGATTCATGTTATAGATATGGACACTATAGA
TGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATTGGAA
GACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAAT
TGCAATGTAGTTCCACATTTCAACAAGATTCAAGATTTTAACGACACTTT
CTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATCGCCA
GAAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGT
GTCTTAGATCCAAGCTCCATTGTCCCTTTGATAGATGGGGGGACAGAAGG
TTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAATGACTGCTTGTATCG
AATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACC
ATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGAT
GTTGCAGTGGCCTAAGGAGCAGCCTTTTGGAGAAGGGGTTCCATTAGATG
GAGATGATCCTGAACATATACAATGGATTTTCCAAAAATCCCTAGAGAGA
GCATCACAATATAATATTAGGGGTGTTACGTATAGGCTCACTCAAGGGGT
AGTAAAAAGAATCATTCCTGCAGTAGCTTCCACAAATGCAGTCATTGCAG
CTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAAGTGCATACATTCCC
TTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACAC
ATTTGAAGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTC
AAAATATTCAGTTTTCTCCATCAGCTAAACTACAGGAGGTTTTGGATTAT
CTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCAGCCATCACAGCCAC
CCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCTATTG
AAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAAGAATTGGGGCTT
GTTGATGGACAAGAACTGGCGGTTGCTGATGTCACCACCCCACAGACTGT
ACTATTCAAACTTCATTTTACTTCTCATCATCACCATCACCATTAAGGAT
CC

DNA sequence of GST APPBP1
CAATTGAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCC
TATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTC
TTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGAT
GAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCC
CAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGG
CCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCA
AAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAG
ATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAG
TTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGT
TTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTT
CATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCC
TGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATC
CCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTT
GCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGG
ATCTGGTTCCGCGTGGATCCATGGCGCAGCTGGGAAAGCTGCTCAAGGAG
CAGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCATGGGCAAGAGGC
TTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTG
AAATTCTTAAAAACTTGGTACTACCAGGTATTGGTTCGTTTACAATTATT
GATGGAAATCAGGTCAGCGGAGAAGATGCTGGAAACAATTTCTTCCTTCA
AAGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTAC
AAGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAA
AACCTTCTAGACAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGT
TGCAACTCAGCTTCCTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCT
GGAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGT
TATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGA
TAATGCATTAGAGGATCTACGACTAGATAAGCCATTTCCTGAACTGAGAG
AACATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAGGACCACAGT
CATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATAG
TGAAACAAATGGACGAATACCTAAAACGTATAAAGAAAAAGAGGACTTCA
GAGATTTGATTAGACAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAA
GATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAACACAGCACTAAA
TACAACTCAGATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCT
GCATAAATATCACCAAACAGACTCCATCATTTTGGATTTTAGCTCGTGCC
TTAAAGGAATTTGTGGCCAAAGAGGGTCAAGGAAATTTACCTGTTCGAGG
CACAATTCCTGATATGATTGCAGATTCAGGCAAATATATAAAACTGCAAA
ACGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCAT
GTTGCCAAATTGCTGCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGA
GAAAGAATTAAAATTACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAA
GATGTCGATCCTTAGCTGAAGAATATGGTTTGGATACAATTAACAAGGAT
GAAATTATTTCTAGCATGGACAATCCAGATAATGAAATAGTGTTGTACTT
AATGTTACGGGCTGTTGATAGATTTCATAAACAACAGGGTAGATATCCAG
GAGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGT
CTCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGA
TTATGTCCACGAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTG
CTGCATTCTTGGGGGGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACC
AAACAATTTGTAATTTTTAATAATACTTACATTTACAGTGGCATGTCACA
AACTTCAGCAACTTTCCAGTTGTAGAAGCTT

Amino Acid Sequence

Protein sequence of UBA3
MAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCK
VLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIG
RPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIA
RRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACI
ECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLD
GDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIA
AVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLP
QNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSI
EERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTSHHHHHH*

Protein sequence of GST APPBP1
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMAQLGKLLKEQKYDRQLRLWGDHG
QEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNF
FLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFT
VVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIES
HPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQ
WYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNT
ALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLP
VRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPES
ISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIV
LYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMV
KDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSG
MSQTSATFQL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pOPC UBA3 6His, GST APPBP12.12 MBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61958
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Correction of DU32498 to add Lac op, LacI and correct premature ATG.; Estimated MW of UBA3 : 50163 Daltons; Estimated MW of GST APPBP1 : 86536 Daltons
• Low Temp: No