cDNA Clone Ubiquitin

• Synonyms: -
• Plasmid: pTXB1 Ubiquitin MGSSSG(R74G delta G75) Mxe intein chitin BD T3C
• Parent Plasmid: pTXB1
• Expressed: N-extended Ub1-74 R74G
• Genbank: See source

Sequence of Insert

CATatgggtTCTTCTTCCGGCcagatcttcgtcaagacgttaaccggtaaaaccataactctagaagtggaaccgagcgataccatcgaaaacgtgaaagcgaaaatccaggataaagaaggcatcccgccggatcagcagcgtctgatctttgcgggcaaacagctggaagatggccgcaccctgtctgattataacatccagaaagagtcgaccctgcatctggtcttaagactgggtTGCATCtgcGGAGATGCACTAGTTGCCCTACCCGAGGGCGAGTCGGTACGCATCGCCGACATCGTGCCGGGTGCGCGGCCCAACAGTGACAACGCCATCGACCTGAAAGTCCTTGACCGGCATGGCAATCCCGTGCTCGCCGACCGGCTGTTCCACTCCGGCGAGCATCCGGTGTACACGGTGCGTACGGTCGAAGGTCTGCGTGTGACGGGCACCGCGAACCACCCGTTGTTGTGTTTGGTCGACGTCGCCGGGGTGCCGACCCTGCTGTGGAAGCTGATCGACGAAATCAAGCCGGGCGATTACGCGGTGATTCAACGCAGCGCATTCAGCGTCGACTGTGCAGGTTTTGCCCGCGGgAAACCCGAATTTGCGCCCACAACCTACACAGTCGGCGTCCCTGGACTGGTGCGTTTCTTGGAAGCACACCACCGAGACCCGGACGCCCAAGCTATCGCCGACGAGCTGACCGACGGGCGGTTCTACTACGCGAAAGTCGCCAGTGTCACCGACGCCGGCGTGCAGCCGGTGTATAGCCTTCGTGTCGACACGGCAGACCACGCGTTTATCACGAACGGGTTCGTCAGCCATGCTACTGGCCTCACCGGTCTGAACTCAGGCCTCACGACAAATCCTGGTGTATCCGCTTGGCAGGTCAACACAGCTTATACTGCGGGACAATTGGTCACATATAACGGCAAGACGTATAAATGTTTGCAGCCCCACACCTCCTTGGCAGGATGGGAACCATCCAACGTTCCTGCCTTGTGGCAGCTTCAATGACTGCAGGAAGGGGATCC

Amino Acid Sequence

MGSSSGQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLGCICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pTXB1 Ubiquitin MGSSSG(R74G delta G75) Mxe intein chitin BD T3C606.23 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71113
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GSSSG sequence inserted after initiating methionine in DU65889 (translated product starts MGSSSGQIFV......); 1bp deletion in initiating Met corrected with NW7960/61
• Low Temp: No