cDNA Clone UBE2A

• Synonyms: UBC2, HHR6A, MRXSN, RAD6A, MRXS30, ubiquitin-conjugating enzyme E2 A
• Plasmid: pET15b-6HIS_TEV UBE2A V39A isoform 1
• Parent Plasmid: pET15b-6HIS_TEV
• Expressed: 6His-UBE2A V39A isoform 1
• Genbank: Gene ID 7319 NM_003336.3 → NP_003327.2 P49459
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGgcCATTTTCGGGCCTGAAGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCCAAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAAGCTGGCGTGATTGTTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGENLYFQGHMLGSPEFPGTVDSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAAIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b-6HIS_TEV UBE2A V39A isoform 1163.85 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71098
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: no native initiating methionine.; Ser41 of fusion = Ser2 of UBE2A; Parental 23047
• Low Temp: No