cDNA Clone UBR4

• Plasmid: pFBDM-UBR4-6His
• Parent Plasmid: pFBDM
• Expressed: UBR4-6His
• Genbank: 23352
• Tag: 6His
• Species: Human

Sequence of Insert

see attached Snapgene file

Amino Acid Sequence

see attached Snapgene file

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDM-UBR4-6His2.29 MBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71056
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental pFBDM. C-ter 6His first added to 65965 with NW7854/55, then N-ter 6His deleted with NW7856/57; ; Clone contains the following changes compared to NCBI ref: Silent changes at Leu3327 (CTG > CTC), Val4039 (GTT > GTC), Lys 4793 (AAG > AAA) and His4959 (CAT>CAC). Listed SNP at Met4867Leu (ATG>TTG) and a non-silent mutation at Leu3797Pro (CTG>CCG). Grow at or below 30ºC
• Low Temp: Yes