cDNA Clone ULK1

• Synonyms: -
• Plasmid: pNIC28 6His sumo TEV ULK1 kinase domain (M1-S283) E37A K38A (P220T)
• Parent Plasmid: pNIC28a
• Expressed: 6His sumo TEV ULK1 kinase domain (M1-S283) E37A K38A (P220T)
• Genbank: NM_003565.2 with changes E37A K38A
• Tag: 6His
• Species: Human

Sequence of Insert

See snapgene file

Amino Acid Sequence

See snapgene file

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pNIC28 6His sumo TEV ULK1 kinase domain (M1-S283) E37A K38A (P220T)173.15 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU70002
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This clone has unwanted mutation P220T. Cannot correct in this plasmid without other mutations arising. Subcloning into final destination plasmid (pETDuet lamdba phosphatase) and will attempt to correct in that plasmid.
• Low Temp: No