cDNA Clone Ufc1 (mouse)

• Synonyms: 1110021H02Rik, AA062310, ESTM29
• Plasmid: pGEX6P1 UFC1 (KO) C116S Mouse
• Parent Plasmid: pGEX6P1
• Expressed: GST C3 Ufc1(K0) C116S
• Genbank: NM_025388.2
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAGGACCAACGCCGGACCCCGAGATCGGGAATTGTGGGTGCAGCGACTAAGGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAGGAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAGGGAAGGGACCCGGTGGTTTGGAAGATGCTGGTACATCCACGATTTCCTCAGATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTGCTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAGGACAGCAAGGATGTACAGGGGTGGCAGAATATCTCTGACTGATCATTTCAGACCTTTGTGGGCCAGGAATGTGCCTAGGTTTGGACTAGCTCATCTCATGGCCCTGGGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAGGGGTGTGATCCAGCACAGAGAAAGATGCAGCCAATGAGCGGCCGCA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLRTNAGPRDRELWVQRLREEYQSLIRYVENNRNSDNDWFRLESNREGTRWFGRCWYIHDFLRYEFDIEFEIPITYPTTAPEIAVPELDGRTARMYRGGRISLTDHFRPLWARNVPRFGLAHLMALGLGPWLAVEVPDLIQRGVIQHRERCSQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 UFC1 (KO) C116S Mouse76.65 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU68504
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kindly mutate all lysine in Ufc1 to Arginine. (K17R, K33R , K47R ,K60R ,K68R ,K77R ,K105R ,K108R ,K114R ,K122R ,K131R ,K156R ,K162R ,K164R)
• Low Temp: No