cDNA Clone UBE3A

• Plasmid: pGEX TEV UBE3A C843A
• Parent Plasmid: pGEX TEV
• Expressed: GST-TEV-UBE3A C843A
• Genbank: Uniprot: Q05086, NCBI: NM_000462.3
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAGAAGCTGCACCAGTGTTATTGGAAATCAGGAGAACCTCA
GTCTGACGACATTGAAGCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATC
TAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCC
TGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAA
TAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAAC
TCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAG
AACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAA
GAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGA
AGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCT
TTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACA
GAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTC
AAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGT
TCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGG
TGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATG
TGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCT
AATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACC
TAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTA
ATTATCTGAATTTGTTCATTATCGTAATGGAGAATAGAAATCTCCACAGT
CCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAA
GCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACA
ATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACT
TATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGA
TGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAA
ATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAA
GAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGA
AGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAAC
TTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAG
TTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATAC
TTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCT
TTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGA
ATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCA
AGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATA
TCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCT
GCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGT
TGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAA
TCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTG
TTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGAT
TGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTAC
ATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTT
CGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTT
ATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGA
TATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAA
AATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAA
TCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGG
CTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTAC
TTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCT
AGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCA
GGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACA
GATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGC
ACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCC
CAGACACAGAAAGGTTACCTACATCTCATACTgcCTTTAATGTGCTTTTA
CTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGC
CATCACGTATGCCAAAGGATTTGGCATGCTGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSMEKLHQCYWKSGEPQSDDIEA
SRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIK
ALELYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARID
FKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVK
QHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGD
NNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDL
TYHNVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQ
GKLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAA
SKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKK
GPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVET
ENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNP
YLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVS
KEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGL
AIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGN
VEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYI
LNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALE
ETTEYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLG
KLKMIIAKNGPDTERLPTSHTAFNVLLLPEYSSKEKLKERLLKAITYAKG
FGML*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65473
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Transcript Variant 2
• Low Temp: No