cDNA Clone UBE2O

• Synonyms: E2-230K
• Plasmid: pcDNA5D-FRT/TO HA-UBE2O
• Parent Plasmid: pcDNA5D-FRT/TO HA
• Expressed: HA-UBE2O
• Genbank: NM_022066.4 → NP_071349.3
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGGCTGATCCCGCAGCCCCCACGCCCGCAGCTCCCGCTCCAGCCCAGGC
CCCGGCTCCAGCCCCGGAGGCAGTCCCGGCCCCAGCCGCAGCCCCCGTCC
CGGCGCCGGCGCCCGCCTCGGACTCGGCCTCCGGGCCGTCCTCGGACTCC
GGCCCAGAAGCCGGCTCGCAGCGCCTGCTGTTTTCTCACGACCTGGTGTC
GGGCCGTTACCGTGGCTCCGTGCACTTCGGGCTGGTGCGCCTCATCCACG
GCGAGGACTCGGACTCGGAGGGCGAGGAGGAGGGCCGCGGGAGCTCGGGG
TGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCCCT
GCGCCGCGGCTACGTGCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGC
ATGTGAAGGAGACCAAGCTGAAACTAGAGGACCGTTCTGTGGTGCCCCGA
GATGTGGTCCGGCACATGCGATCCACCGACAGTCAGTGTGGCACGGTGAT
CGACGTCAACATCGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCA
TCTATCCCGTCAACAGCAAGGACCTGCAGCACATCTGGCCCTTCATGTAT
GGGGACTACATTGCCTATGACTGCTGGCTGGGGAAGGTCTACGACTTGAA
GAACCAGATCATCCTGAAGCTATCCAACGGCGCCAGGTGCTCCATGAACA
CGGAAGATGGCGCCAAGCTCTACGACGTCTGCCCGCACGTCAGCGACTCG
GGTCTCTTCTTCGATGATTCCTATGGCTTCTACCCAGGCCAGGTGCTCAT
TGGCCCTGCCAAGATCTTCTCCAGCGTCCAGTGGCTGTCAGGTGTCAAGC
CCGTGCTCAGCACCAAGAGCAAGTTCCGAGTGGTGGTGGAAGAGGTGCAG
GTTGTAGAGTTGAAAGTTACATGGATTACCAAGAGTTTCTGTCCAGGGGG
CACGGACAGCGTCAGCCCCCCACCCTCTGTCATCACCCAGGAAAACCTAG
GCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGG
GAGCGCTGTCTGTATGTCTTCCCAGCCAAGGTAGAGCCAGCCAAGATTGC
CTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGGAGGGCTCTATGGCCA
AGAAGGTGAAGCGCCTGTTGAAGAAGCAGGTTGTGCGGATCATGTCATGC
TCCCCAGACACCCAGTGTTCCCGGGACCATTCCATGGAAGACCCAGACAA
GAAGGGGGAATCCAAAACCAAGAGCGAAGCGGAGTCTGCCAGCCCTGAGG
AGACGCCCGATGGCTCTGCCAGTCCAGTGGAGATGCAGGACGAGGGTGCA
GAGGAGCCCCACGAGGCAGGAGAGCAGCTGCCCCCATTCCTGCTAAAAGA
AGGCAGAGATGACAGGCTGCACTCGGCAGAGCAGGACGCAGATGATGAGG
CTGCTGATGACACGGACGACACCAGTTCGGTGACCTCCTCTGCCAGCTCC
ACCACTTCCTCCCAGAGCGGCAGCGGCACGAGTCGCAAAAAGAGCATCCC
CTTGTCCATCAAGAACTTAAAGCGCAAACACAAGAGGAAGAAGAATAAAA
TCACTCGAGACTTCAAGCCAGGGGACAGGGTGGCAGTGGAGGTGGTGACC
ACGATGACCTCAGCCGACGTGATGTGGCAGGATGGCTCCGTGGAATGCAA
CATCCGCTCCAACGACCTCTTCCCTGTGCACCACCTGGACAACAACGAGT
TCTGCCCTGGAGACTTCGTGGTAGATAAGCGAGTCCAGAGCTGTCCAGAC
CCTGCTGTCTACGGTGTGGTACAGTCTGGGGACCACATCGGCCGTACCTG
CATGGTGAAGTGGTTCAAGCTGAGGCCGAGTGGGGACGACGTGGAGCTGA
TTGGAGAAGAGGAAGATGTGAGTGTTTACGACATTGCTGACCACCCTGAC
TTTAGGTTCCGTACAACTGACATCGTCATCCGCATCGGCAATACTGAGGA
TGGGGCTCCTCACAAGGAGGATGAGCCATCGGTGGGCCAGGTGGCCCGTG
TGGACGTCAGCAGCAAGGTGGAGGTGGTGTGGGCTGACAACTCAAAGACC
ATCATCCTGCCCCAGCACTTGTACAACATAGAGTCTGAGATTGAGGAGTC
AGACTACGATTCGGTAGAAGGCAGCACCAGCGGGGCATCCTCGGATGAAT
GGGAAGATGATAGTGACAGCTGGGAGACGGACAATGGGCTGGTGGAGGAC
GAGCACCCCAAGATAGAGGAGCCCCCCATCCCACCCCTGGAGCAGCCGGT
GGCCCCTGAGGACAAGGGAGTGGTGATCAGTGAAGAGGCAGCCACAGCTG
CCGTCCAGGGGGCTGTGGCCATGGCTGCCCCCATGGCCGGGCTGATGGAG
AAGGCTGGCAAGGACGGGCCACCCAAGAGCTTCCGGGAGTTGAAAGAGGC
CATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTGA
CGGGCTCGCCCACCTCTCCGACTGTGGAGCCTGAGAAGCCAACTCGGGAG
AAGAAGTTTCTGGATGACATCAAGAAGCTACAGGAAAACCTCAAGAAGAC
CCTGGACAATGTGGCCATTGTAGAGGAGGAGAAGATGGAAGCAGTGCCCG
ACGTAGAGCGCAAGGAGGACAAGCCCGAGGGGCAGTCACCTGTGAAGGCT
GAGTGGCCCAGCGAAACCCCGGTGCTGTGCCAGCAGTGTGGCGGCAAGCC
TGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCCGTACTGGAGT
TTGCACCCTCAAATCATTCTTTTAAGAAAATTGAGTTCCAGCCTCCAGAA
GCCAAGAAGTTCTTCAGCACAGTGCGGAAGGAGATGGCGCTGCTGGCTAC
CTCACTGCCTGAGGGCATCATGGTCAAGACTTTTGAAGATAGAATGGACC
TCTTCTCAGCTCTCATCAAGGGCCCCACTCGAACCCCCTACGAGGATGGC
CTCTACTTGTTTGACATCCAGCTCCCCAACATCTACCCAGCCGTGCCCCC
CCACTTCTGCTACCTCTCCCAATGCAGTGGCCGCCTGAACCCCAACCTGT
ATGACAATGGGAAGGTGTGTGTCAGCCTCCTGGGCACCTGGATTGGAAAG
GGGACAGAGAGGTGGACAAGCAAGTCCAGCCTTCTCCAGGTGCTCATCTC
CATCCAAGGTCTGATCCTGGTAAATGAACCATACTACAACGAAGCCGGCT
TCGACAGTGACCGAGGCCTGCAGGAAGGCTATGAAAACAGTCGCTGTTAC
AATGAGATGGCGCTGATCCGCGTGGTGCAGTCCATGACCCAGCTGGTGCG
GCGGCCCCCCGAGGTCTTTGAGCAGGAGATCAGGCAACACTTTAGCACTG
GTGGCTGGCGGCTGGTGAACCGTATCGAGTCCTGGCTGGAAACCCATGCC
CTGCTGGAGAAGGCCCAGGCACTGCCCAACGGGGTGCCCAAGGCCAGCAG
CTCGCCAGAGCCCCCAGCTGTAGCCGAGCTGTCAGACTCCGGCCAACAAG
AACCTGAGGATGGAGGGCCAGCCCCAGGAGAGGCCTCCCAGGGCTCAGAC
TCAGAGGGCGGTGCCCAGGGCCTGGCCTCAGCTAGCAGGGACCACACAGA
CCAGACTTCGGAGACCGCACCAGACGCATCGGTGCCACCCAGTGTGAAAC
CAAAGAAGCGGAGAAAGAGCTACCGGAGCTTCTTACCTGAGAAGAGTGGC
TACCCTGACATCGGCTTCCCCCTCTTCCCACTTTCCAAGGGTTTCATCAA
GAGCATCCGGGGTGTCCTGACGCAGTTCCGGGCTGCCCTGCTAGAGGCAG
GCATGCCGGAGTGCACAGAGGACAAGTAGgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAP
ASDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSD
SEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKET
KLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVN
SKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGA
KLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLST
KSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKR
LGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKR
LLKKQVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDG
SASPVEMQDEGAEEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDT
DDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDF
KPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGD
FVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDDVELIGEEE
DVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSS
KVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDS
DSWETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGA
VAMAAPMAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPT
SPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIVEEEKMEAVPDVERK
EDKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSN
HSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSAL
IKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGK
VCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDR
GLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRL
VNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDG
GPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRR
KSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPEC
TEDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65398
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41456; Fwd amplification oligo introduced silent change compared to NCBI ref at Ala2 (GCG > GCT) to kill internal BamHI just in from start codon
• Low Temp: No