cDNA Clone ubiquitin

• Plasmid: pET15-M1-GCSSG-Q2-UBIQUITIN-6HIS TAG40
• Parent Plasmid: pET15
• Expressed: MGCSSG-ubiquitin-His TAG40
• Tag: 6His
• Species: Human

Amino Acid Sequence

MGCSSGQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPD*QRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRGGHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29981
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Proximity to upstream shine Dalgarno sequence restored compared to 29958 GCSSG inserted between M1 and Q2 of ubiquitin ORF. C-terminal Hisx6 tag immediately follows GG (retain TAA stop codon).
• Low Temp: No