cDNA Clone VRP1/YLR337C

• Plasmid: pRS413 ADH Pr 400-VRP1/YLR337C-200
• Parent Plasmid: pRS413 ADH Pr
• Expressed: VRP1/YLR337C
• Genbank: (ID:851051)

Sequence of Insert

GGATCCTCCGCTACATTGCTGTATTCTCTTTTAAGTTCAAATTACAAGGGCAAGATAAAAATTTTAGCTCACAAAAGATGGAAAAAGTCGCAAATATTCAAGTTACGAAACATTGACAGAGGATGGAATCACAATGTTAGCCTTCAAATCCTACTATCCTGTATGAAGCTTGCTAAAAGCGTCTTTAGAAAGAATGAAATGTTCATAACCTAGTCACTGCTTACGGGTGCGGCCGCCGTCCTCAAATAGGGGCCTTAAGAATAATCCGCACAGTCAGTAAATACTTGAACAAAATCTAAAATTATAAACTTATGAAGAAAGTTTTTGATATGAGACAAAGCATACGACTAAAAAGCGCACAACCGTTTGTTGAAGAAGCCTACCATATATATAACGAATTGTTGAAATGGCAGGTGCTCCAGCTCCTCCTCCTCCACCGCCTCCACCAGCATTAGGTGGTAGTGCTCCTAAACCGGCAAAATCTGTGATGCAGGGCCGTGACGCCCTGTTAGGTGACATTAGAAAGGGAATGAAACTAAAGAAAGCTGAGACCAATGATAGAAGTGCCCCAATCGTGGGAGGTGGTGTCGTTTCATCGGCCTCAGGAAGTTCTGGTACCGTCAGTAGTAAAGGACCCTCTATGTCAGCTCCTCCAATCCCTGGGATGGGCGCTCCGCAGTTGGGCGATATTTTAGCTGGAGGTATACCGAAGTTAAAACATATCAATAACAATGCAAGCACTAAACCCTCTCCATCGGCGAGCGCACCGCCAATTCCGGGCGCGGTACCATCTGTTGCGGCTCCCCCCATTCCTAATGCACCATTATCACCAGCACCCGCTGTTCCTAGTATTCCCTCGTCTTCTGCACCTCCAATTCCTGATATTCCATCTTCTGCGGCCCCTCCTATTCCCATTGTACCATCATCTCCAGCACCACCGCTTCCATTATCAGGTGCCTCTGCTCCAAAGGTACCGCAAAATAGACCGCATATGCCTTCAGTAAGGCCAGCACACAGAAGCCACCAAAGAAAAAGCTCTAATATTTCTCTTCCTAGTGTGTCAGCACCCCCTTTGCCTTCAGCGTCTCTGCCAACACATGTTAGCAACCCTCCACAAGCTCCACCCCCTCCCCCAACTCCAACAATTGGCCTGGACTCAAAAAACATTAAACCTACAGATAACGCAGTCTCACCACCATCTTCTGAAGTTCCTGCAGGCGGGTTGCCATTTTTGGCAGAAATTAATGCCAGGAGGTCTGAACGAGGAGCCGTCGAGGGCGTTTCTTCCACAAAGATTCAAACCGAAAATCATAAATCTCCTTCACAACCACCACTTCCATCATCTGCTCCGCCCATTCCTACATCACATGCTCCGCCATTACCGCCTACTGCACCACCTCCACCATCGTTACCAAATGTGACCTCTGCACCTAAAAAAGCAACTTCAGCTCCAGCTCCGCCTCCACCACCACTACCAGCAGCCATGAGCTCTGCGTCGACTAACAGCGTAAAAGCAACCCCGGTTCCCCCAACTTTAGCACCACCGTTACCAAATACTACATCAGTTCCTCCTAACAAAGCATCGTCAATGCCCGCGCCACCACCTCCTCCTCCCCCACCACCTGGTGCTTTTTCAACATCCTCTGCCTTGTCAGCATCATCTATTCCTTTAGCACCACTACCACCTCCTCCTCCTCCTTCGGTTGCAACGTCAGTACCATCGGCTCCTCCTCCTCCAACACTCACGACAAACAAACCCTCAGCGTCCAGTAAGCAATCCAAAATATCTTCATCCAGTTCTTCCTCGGCCGTAACACCTGGGGGACCCTTACCATTTCTTGCTGAGATCCAAAAGAAGAGAGATGATAGATTCGTGGTTGGTGGTGATACTGGATACACTACCCAAGATAAACAAGAGGATGTAATAGGTTCATCTAAGGATGATAACGTAAGACCTTCTCCTATTTCTCCATCTATAAACCCGCCTAAACAGAGTAGCCAAAATGGTATGTCGTTTCTGGATGAAATTGAATCCAAATTACATAAGCAAACATCATCGAATGCTTTTAACGCACCACCCCCCCATACGGATGCTATGGCGCCTCCTTTGCCTCCTAGTGCACCTCCTCCTCCTATAACATCGCTACCGACTCCAACTGCATCAGGTGATGACCACACCAATGATAAGAGTGAGACTGTGTTAGGGATGAAAAAAGCAAAGGCCCCTGCACTGCCTGGACACGTTCCACCTCCACCAGTTCCACCAGTTTTATCAGATGATAGCAAGAATAATGTCCCCGCCGCTTCTTTATTACACGATGTTCTTCCCTCCTCGAATCTCGAAAAACCTCCTTCTCCTCCTGTTGCAGCTGCTCCGCCATTGCCAACATTTAGTGCACCAAGTCTGCCTCAGCAATCCGTGTCTACATCAATACCATCACCTCCTCCTGTTGCGCCTACACTTTCCGTTCGTACCGAAACTGAATCAATTTCGAAGAACCCCACTAAGTCGCCACCCCCACCACCAAGTCCATCTACGATGGATACCGGTACCTCTAATAGCCCCAGTAAAAACCTTAAACAACGGTTATTTTCTACAGGTGGATCGACGCTGCAACACAAGCATAATACGCATACAAATCAACCAGATGTTGATGTAGGTAGGTATACGATAGGGGGATCGAATTCTATCGTTGGGGCCAAATCTGGAAATGAAAGAATTGTTATAGATGATTCAAGATTTAAATGGACTAATGTGTCACAAATGCCTAAGCCACGTCCCTTTCAAAATAAAACAAAACTGTATCCAAGTGGAAAGGGTAGTAGTGTGCCATTGGACTTAACATTATTTACGTGAGCATTTCTCCATGGTTACAATAAATCACTGAAGAACAAGAAAATAATAATATTTTATAAAAATATTTTACTAAATTACATATGGTATCTGCAATATTTATTTTTTAATCTTTTAGCTTCCCGTCCTTTTCGCTGTGAAAAATATAAAATCCAGCAAAAAAAAAAAAATGCGATGAGCTTACAAGACTACTGAACTTACTTCTCGAG

Amino Acid Sequence

MAGAPAPPPPPPPPALGGSAPKPAKSVMQGRDALLGDIRKGMKLKKAETNDRSAPIVGGGVVSSASGSSGTVSSKGPSMSAPPIPGMGAPQLGDILAGGIPKLKHINNNASTKPSPSASAPPIPGAVPSVAAPPIPNAPLSPAPAVPSIPSSSAPPIPDIPSSAAPPIPIVPSSPAPPLPLSGASAPKVPQNRPHMPSVRPAHRSHQRKSSNISLPSVSAPPLPSASLPTHVSNPPQAPPPPPTPTIGLDSKNIKPTDNAVSPPSSEVPAGGLPFLAEINARRSERGAVEGVSSTKIQTENHKSPSQPPLPSSAPPIPTSHAPPLPPTAPPPPSLPNVTSAPKKATSAPAPPPPPLPAAMSSASTNSVKATPVPPTLAPPLPNTTSVPPNKASSMPAPPPPPPPPPGAFSTSSALSASSIPLAPLPPPPPPSVATSVPSAPPPPTLTTNKPSASSKQSKISSSSSSSAVTPGGPLPFLAEIQKKRDDRFVVGGDTGYTTQDKQEDVIGSSKDDNVRPSPISPSINPPKQSSQNGMSFLDEIESKLHKQTSSNAFNAPPPHTDAMAPPLPPSAPPPPITSLPTPTASGDDHTNDKSETVLGMKKAKAPALPGHVPPPPVPPVLSDDSKNNVPAASLLHDVLPSSNLEKPPSPPVAAAPPLPTFSAPSLPQQSVSTSIPSPPPVAPTLSVRTETESISKNPTKSPPPPPSPSTMDTGTSNSPSKNLKQRLFSTGGSTLQHKHNTHTNQPDVDVGRYTIGGSNSIVGAKSGNERIVIDDSRFKWTNVSQMPKPRPFQNKTKLYPSGKGSSVPLDLTLFT*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59830
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of VRP1 including 400bp of the 5'UTR and 200bp of the 3'UTR in the vector pRS413-ADH-Pr, changes to reference sequence: silent g781a and del P444
• Low Temp: No