cDNA Clone Ubiquitin

• Plasmid: pET24-Ubiquitin Thr12Ser
• Parent Plasmid: pET24a
• Expressed: Ubiquitin [Thr12Ser]
• Genbank: P62988
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGtCCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKSITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU29928
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA) Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389). T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.
• Low Temp: No