cDNA Clone Ufc1

• Plasmid: pGEX6P1 Ufc1(ms) C116A
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Ufc1(ms) C116A
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAAGACCAACGCCGGACCCCGAGATCGGGAATTGTGGGTGCAGCGACTAAAGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAAGAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAAGGAAGGGACCCGGTGGTTTGGAAAATGCTGGTACATCCACGATTTCCTCAAATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTGCTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAAGACAGCAAAGATGTACAGGGGTGGCAAAATAGCTCTGACTGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCTAAGTTTGGACTAGCTCATCTCATGGCCCTGGGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAAGGGTGTGATCCAGCACAAAGAAAAATGCAGCCAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNSDNDWFRLESNKEGTRWFGKCWYIHDFLKYEFDIEFEIPITYPTTAPEIAVPELDGKTAKMYRGGKIALTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEVPDLIQKGVIQHKEKCSQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59458
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate Cysteine 116 to Alanine
• Low Temp: No